Arch Plast Surg.  2021 Jul;48(4):448-456. 10.5999/aps.2020.00997.

Freeze-dried bovine amniotic membrane as a cell delivery scaffold in a porcine model of radiation-induced chronic wounds

Affiliations
  • 1Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine, Daegu, Korea
  • 2Department of Radiation Oncology, Keimyung University School of Medicine, Daegu, Korea
  • 3Department of Pathology, Keimyung University School of Medicine, Daegu, Korea

Abstract

Background
Locoregional stem cell delivery is very important for increasing the efficiency of cell therapy. Amnisite BA (Amnisite) is a freeze-dried amniotic membrane harvested from bovine placenta. The objective of this study was to investigate the retention of cells of the stromal vascular fraction (SVF) on Amnisite and to determine the effects of cell-loaded Amnisite in a porcine radiation-induced chronic wound model.
Methods
Initially, experiments were conducted to find the most suitable hydration and incubation conditions for the attachment of SVF cells extracted from pig fat to Amnisite. Before seeding, SVFs were labeled with PKH67. The SVF cell-loaded Amnisite (group S), Amnisite only (group A), and polyurethane foam (group C) were applied to treat radiation-induced chronic wounds in a porcine model. Biopsy was performed at 10, 14, and 21 days post-operation for histological analysis.
Results
Retaining the SVF on Amnisite required 30 minutes for hydration and 1 hour for incubation. A PKH67 fluorescence study showed that Amnisite successfully delivered the SVF to the wounds. In histological analysis, group S showed increased re-epithelialization and revascularization with decreased inflammation at 10 days post-operation.
Conclusions
SVFs had acceptable adherence on hydrated Amnisite, with successful cell delivery to a radiation-induced chronic wound model.

Keyword

Amniotic membrane / Cell therapy / Tissue scaffold / Wound healing / Experimental animal model
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