Nat Prod Sci.  2021 Mar;27(1):49-53. 10.20307/nps.2021.27.1.49.

Phenolic Constituents from Balanophora laxiflora with their Anti-inflammatory and Cytotoxic Effects

• Hong Anh NT
• Duong NT
• Vinh PD
• Ha DT

• KMID: 2514910
• DOI: http://doi.org/10.20307/nps.2021.27.1.49

Abstract

Balanophora laxiflora Hemsl. (Balanophoraceae) is a traditional medicinal plant with a diverse array of biological activities. In our exploration of new bioactive constituents from B. laxiflora, we isolated five compounds, including a new lignan, balanophorone (5), and four known phenolic compounds (1–4). The chemical structures of these compounds were determined by extensive spectroscopic analyses, including 1D and 2D NMR, HR-ESI-MS, and CD. In addition, we evaluated the effects of each of the isolates (1–5) on the messenger RNA expression levels of tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells. Compound 2 showed significant inhibition of LPS-induced COX-2 and TNF-α expression in RAW 264.7 macrophages, while compound 4 showed moderate cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells, with IC ₅₀ values of 18.3 and 30.7 μM, respectively. No significant effects on the viability of normal mammary epithelial cells were observed.

Keyword

Balanophora laxiflora; Balanophorone; Anti-inflammation; COX-2; TNF-α; Cytotoxicity