Lab Med Online.  2020 Oct;10(4):326-329. 10.47429/lmo.2020.10.4.326.

A Case of Partial D Type VI-3 Confirmed by RHD Genotyping

  • 1Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
  • 2Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, Korea


Weak D and partial D result in quantitative and qualitative changes in RhD protein expression respectively. It is difficult to discriminate weak D from partial D by serological tests alone. RHD genotyping is a useful method that complements serological results. A 64-year-old woman visited our hospital for microvascular decompression surgery. Her blood type was O, D negative by manual tube test and as per auto analyzer results (QWALYS-3 system; DIAGAST, France). Weak D and partial D tests were performed by using two different monoclonal anti-D reagents (Bioscot; Merck Millipore, UK; Bioclone; Ortho Clinical Diagnostics, USA) and a panel of nine monoclonal antibodies, including anti-D IgM and IgG (D-Screen; DIAGAST, France). However, these serological tests could not confirm the subtype of partial D. Therefore, sequencing of RHD exon 1 to 10 was additionally performed for the patient and the case was revealed to be partial DVI type 3.


RhD; Weak D; Partial D; Genotyping; Sequencing


  • Fig. 1 The blue boxes and the white boxes represent RHD and RHCE exons 1-10, respectively. The arrows indicate negative PCR amplification of RHCE exons 3-6, which suggests RHD*DVI.3.


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