Int J Stem Cells.  2020 Nov;13(3):404-413. 10.15283/ijsc20052.

Upregulation of C-Reactive Protein by Placenta-Derived Mesenchymal Stem Cells Promotes Angiogenesis in A Rat Model with Cirrhotic Liver

Affiliations
  • 1Department of Biomedical Science, CHA University, Seongnam, Korea
  • 2Non-Clinical Evaluation Center, CHA Advanced Research Institute, Seongnam, Korea
  • 3Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea
  • 4Department of Internal Medicine, Catholic University Medical College, Seoul, Korea

Abstract

Background and Objectives
Liver cirrhosis is accompanied by abnormal vascular shunts. The Wnt pathway is essential for endothelial cell survival and proliferation. C-reactive protein (CRP), which is produced by hepatocyte, activates angiogenesis in cardiovascular diseases.
Methods and Results
The expression of CRP in CCl 4 -injured rat livers was detected using qRT-PCR and Western blotting after transplantation of placenta-derived mesenchymal stem cells (PD-MSCs) into rats. To determine whether CRP functions in hepatic regeneration by promoting angiogenesis through the Wnt pathway, we detected VEGF and β-catenin in liver tissues and BrdU and β-catenin in hepatocytes by immunofluorescence. The expression levels of CRP, Wnt pathway-related and angiogenic factors were increased in CCl 4 -injured and PD-MSCs transplanted rat livers. In vitro, the expression levels of Wnt signaling and angiogenic factors were decreased in siRNA-CRP-transfected rat hepatocytes.
Conclusions
CRP upregulation by PD-MSCs participates in vascular remodeling to promote liver regeneration via the Wnt signaling pathway during hepatic failure.

Keyword

Liver cirrhosis; C-reactive protein; Placenta-derived mesenchymal stem cells; Wnt pathway; Vascular remodeling
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