Clin Exp Reprod Med.  2020 Sep;47(3):207-212. 10.5653/cerm.2020.03370.

Association of GRIA1 polymorphisms with ovarian response to human menopausal gonadotropin in Iranian women

• Golestanpour H
• Javadi G
• Sheikhha MH

• KMID: 2506146
• DOI: http://doi.org/10.5653/cerm.2020.03370

Abstract

Objective
Glutamate ionotropic receptor AMPA type subunit 1 (GRIA1) is a subunit of a ligand-gated ion channel that regulates the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) by controlling the release of gonadotropin-releasing hormone. Few studies have investigated the association between the GRIA1 gene and human infertility. This study evaluated the association of the GRIA1 rs548294 C>T and rs2195450 G>A polymorphisms with the ovarian response to human menopausal gonadotropin (HMG) in Iranian women.
Methods
One hundred women with histories of at least 1 year of infertility were included. On the second day of menstruation, patients were injected with HMG; on the third day, blood samples were collected. After hormonal analysis, the GRIA1 rs548294 C >T and rs2195450 G >A genotypes of samples were identified via the restriction fragment length polymorphism method, and on day 9, the number of follicles was assessed via ultrasound.
Results
For the GRIA1 rs548294 C>T and rs2195450 G>A single nucleotide polymorphisms, the subjects with CT and GG genotypes, respectively, displayed the highest mean FSH level, LH level, and number of follicles on day 9 of the menstrual cycle (p <0.05). Significant positive correlations were observed between LH and FSH (p <0.01), LH and follicle count (p <0.01), FSH and age (p <0.05), follicle count and age (p=0.048), and FSH and follicle count (p<0.01).
Conclusion
This study showed a significant relationship between GRIA1 polymorphisms and ovarian response to the induction of ovulation. Therefore, determining patients’ GRIA1 genotype may be useful for improving treatment and prescribing suitable doses of ovulation-stimulating drugs.

Keyword

GRIA1; Ovarian stimulation; Polymorphism; Restriction fragment length polymorphism