Lab Anim Res.  2020 Jun;36(2):88-97. 10.1186/s42826-020-00044-2.

Highlights on selected microscopy techniques to study zebrafish developmental biology

  • 1Clinical Laboratory Sciences Department, College of Applied Medical Science, Taif University, Taif, Kingdom of Saudi Arabia
  • 2Department of Biomedical Engineering, School of Applied Medical Sciences, German Jordanian University, Amman, Jordan


Bio-imaging is a tedious task when it concerns exploring cell functions, developmental mechanisms, and other vital processes in vivo. Single-cell resolution is challenging due to different issues such as sample size, the scattering of intact and opaque tissue, pigmentation in untreated animals, the movement of living organs, and maintaining the sample under physiological conditions. These factors might lead researchers to implement microscopy techniques with a suitable animal model to mimic the nature of the living cells. Zebrafish acquired its prestigious reputation in the biomedical research field due to its transparency under advanced microscopes. Therefore, various microscopy techniques, including Multi-Photon, Light-Sheet Microscopy, and Second Harmonic Generation, simplify the discovery of different types of internal functions in zebrafish. In this review, we briefly discuss three recent microscopy techniques that are being utilized because they are non-invasive in investigating developmental events in zebrafish embryo and larvae.


Zebrafish; Development; Animal model; Two-Photon Microscopy; Second Harmonic Generation; Light-Sheet Microscopy
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