J Korean Assoc Oral Maxillofac Surg.  2013 Aug;39(4):150-155.

Osteogenic potential of adult stem cells from human maxillary sinus membrane by Simvastatin in vitro: preliminary report

• Yun KI
• Kim DJ
• Park JU

Abstract

OBJECTIVES
The objective of this study is to determine the adequate concentration and to evaluate the osteogenic potential of simvastatin in human maxillary sinus membrane-derived stem cells (hSMSC).
MATERIALS AND METHODS
Mesenchymal stem cells derived from the human maxillary sinus membrane were treated with various concentrations of simvastatin. The adequate concentration of simvastatin for osteogenic induction was determined using bone morphogenetic protein (BMP-2). The efficacy of osteogenic differentiation of simavastatin was verified using osteocalcin mRNA, and the mineralization efficacy of hSMSCs and simvastatin treatment was compared with alkaline phosphatase and von Kossa staining.
RESULTS
Expression of BMP-2 mRNA and protein was observed after three days and was dependent on the concentration of simvastatin. Expression of osteocalcin mRNA was observed after three days in the 1.0 microM simvastatin-treated group. Mineralization was observed after three days in the simvastatin-treated group.
CONCLUSION
These results suggest that simvastatin induces the osteogenic potential of mesenchymal stem cells derived from the human maxillary sinus membrane mucosa.

Keyword

Adult stem cells; Maxillary sinus; Simvastatin

MeSH Terms

Adult
Adult Stem Cells
Alkaline Phosphatase
Bone Morphogenetic Proteins
Durapatite
Humans
Maxillary Sinus
Membranes
Mesenchymal Stromal Cells
Mucous Membrane
Osteocalcin
RNA, Messenger
Simvastatin
Stem Cells
Alkaline Phosphatase
Bone Morphogenetic Proteins
Durapatite
Osteocalcin
RNA, Messenger
Simvastatin

Figure

• 

  Fig. 1 Effect of simvastatin on bone morphogenetic protein (BMP)-2 mRNA expression in human sinus membrane stem cells using reverse transcriptase-polymerase chain reaction. BMP-2 mRNA levels were measured after cells were stimulated with simvastatin for 72 hours.

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  Fig. 2 Effect of simvastatin on bone morphogenetic protein (BMP)-2 protein expressions in human sinus membrane stem cells using Westen blot analysis. BMP-2 expression was measured after cells were stimulated with simvastatin for 72 hours.

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  Fig. 3 Effect of simvastatin on osteocalcin mRNA expression in human sinus membrane stem cells using reverse transcriptase-polymerase chain reaction. Osteocalcin mRNA levels were measured after cells were stimulated with simvastatin for 72 hours.

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  Fig. 4 Effects of simvastatin (1.0 µM) on alkaline phosphatase expression and mineralization in human sinus membrane stem cells using alkaline phosphatase staining (E to H) and von Kossa staining (I to L) (×200). A-D as control.

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