Ann Lab Med.  2019 Mar;39(2):223-226. 10.3343/alm.2019.39.2.223.

Reconstructed Three-Dimensional Images and Parameters of Individual Erythrocytes Using Optical Diffraction Tomography Microscopy

• Koo SE
• Jang S
• Park Y
• Park CJ

• KMID: 2425981
• DOI: http://doi.org/10.3343/alm.2019.39.2.223

Abstract

No abstract available.

MeSH Terms

Erythrocytes*
Imaging, Three-Dimensional*
Microscopy*

Figure

• 

  Fig. 1 Erythrocyte visualization and measurement of parameters from 3-D RI tomograms: (A) Measuring the diameter directly from the erythrocyte image. (B) Measuring various kinds of parameters calculated from 3-D and 2-D RI tomograms of an erythrocyte. (C) Output of the commercial software used (Tomostudio, Tomocube, Inc., Daejeon, Korea).Abbreviations: 3-D, three-dimensional; 2-D, two-dimensional; CH, corpuscular hemoglobin content; CV, corpuscular volume; CHC, corpuscular hemoglobin concentration; SA, surface area; SI, sphericity.

• 

  Fig. 2 Reconstructed three-dimensional refractive index tomograms of erythrocytes. Upper and side views of a normal erythrocyte (A, B), upper view of a teardrop cell (C), upper and side views of a target cell (D, E), side view of a schistocytes (F, G), an acanthocyte (H), and a burr cell (I). The reconstructed erythrocyte images were colored red by the authors' choice; they were originally greyscale images obtained using a commercial software (Tomostudio, Tomocube, Inc., Daejeon, Korea).

Reference

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