Tissue Eng Regen Med.  2018 Oct;15(5):639-647. 10.1007/s13770-018-0133-y.

Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics

Affiliations
  • 1Biomedical Research Institute, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu 41944, Korea.
  • 2Department of Urology, School of Medicine, Kyungpook National University, 130 Dongdeok-ro, Jung-gu, Daegu 41944, Korea. urokbs@knu.ac.kr, tgkwon@knu.ac.kr
  • 3Department of Urology, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu 41944, Korea.
  • 4Department of Urology, Kyungpook National University Chilgok Hospital, 807 Hogukro, Buk-gu, Daegu 41404, Korea.
  • 5Department of Laboratory Animal Research Support Team, Yeungnam University Medical Center, 170 Hyunchung-ro, Nam-gu, Daegu 42415, Korea.
  • 6Department of Pathology, Central Hospital, 480 Munsu-ro, Nam-gu, Ulsan 44667, Korea.
  • 7Department of Urology, College of Medicine, Yeungnam University, 170 Hyunchung-ro, Nam-gu, Daegu 42415, Korea.

Abstract

BACKGROUND
The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions.
METHODS
To establish optimal conditions, human amniotic fluid stem cells' (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 ℃), cell density (1 × 10⁷ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells.
RESULTS
Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 ℃, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells.
CONCLUSION
Our findings can provide a foundation to optimization of conditions for stem cell transport.

Keyword

Cell transporting; Medium; Cell density; Amniotic fluid stem cell

MeSH Terms

Amniotic Fluid
Cell Count
Cell Survival*
Cell- and Tissue-Based Therapy
Female
Glass
Humans
Stem Cells*
Survival Rate
Syringes
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