J Vet Sci.  2017 Mar;18(1):101-104. 10.4142/jvs.2017.18.1.101.

Stage-specific embryonic antigen: determining expression in canine glioblastoma, melanoma, and mammary cancer cells

  • 1Department of Veterinary Clinical Sciences and Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA. modiano@umn.edu wmlin925@126.com
  • 2Department of Veterinary Medicine, College of Life Sciences, Longyan University, Longyan 364012, China.


The expression of stage-specific embryonic antigens (SSEAs) was determined in several types of canine cancer cells. Flow cytometry showed SSEA-1 expression in glioblastoma, melanoma, and mammary cancer cells, although none expressed SSEA-3 or SSEA-4. Expression of SSEA-1 was not detected in lymphoma, osteosarcoma, or hemangiosarcoma cell lines. Relatively stable SSEA-1 expression was observed between 24 and 72 h of culture. After 8 days in culture, sorted SSEA-1⁻ and SSEA-1⁺ cells re-established SSEA-1 expression to levels comparable to those observed in unsorted cells. Our results document, for the first time, the expression of SSEA-1 in several canine cancer cell lines.


canine cancer; flow cytometry; magnetic-activated cell sorting; stage-specific embryonic antigens

MeSH Terms

Cell Differentiation
Cell Line, Tumor
Dog Diseases
Flow Cytometry/veterinary
Mammary Neoplasms, Animal/etiology/*metabolism
Stage-Specific Embryonic Antigens/*metabolism
Stage-Specific Embryonic Antigens


  • Fig. 1 Stage-specific embryonic antigen (SSEA)-1 expression in canine cancer cells. (A) Flow cytometric analysis of SSEA-1 expression in canine melanoma TLM1, mammary cancer CMT27, and glioblastoma Candy cancer cell lines. Cells were incubated with anti-SSEA-1-PE antibody. A minimum of 10,000 events were collected per sample. Data shown are representative of several independent experiments. (B) SSEA-1 expression in canine mammary cancer CMT83 cells at various culture times. Cells were incubated with anti-SSEA-1-PE antibody. Data are expressed as mean ± SD.

  • Fig. 2 Flow cytometric analysis of stage-specific embryonic antigen (SSEA)-1 expression in mammary cancer CMT83 cell line. The sorted SSEA-1 positive (+) and negative (−) cells were cultured separately and harvested for re-analysis of SSEA-1 expression after 8 days of culture. Values are taken from one of three independent experiments.


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