Clin Exp Vaccine Res.  2017 Jul;6(2):160-163. 10.7774/cevr.2017.6.2.160.

Application of aptamers for assessment of vaccine efficacy

• Yoon SY
• Gee G
• Hong KJ
• Seo SH

• KMID: 2391577
• DOI: http://doi.org/10.7774/cevr.2017.6.2.160

Abstract

Assessing antigen concentration of vaccine is essential step in determining the quality of the vaccine prior to vaccination. After vaccination, vaccine-induced antibody titer should also be measured to verify the vaccine efficacy. Since conventional assay used for vaccine concentrations and induced Ab-titers is antibody-based enzyme-linked immunosorbent assay, the assay inevitably brings drawbacks of antibody such as high cost for production, limited stability, and inconsistent quality between lot-to-lots. Aptamer is single-stranded nucleic acid having three-dimensional structure and has features overcoming limitations of antibody. This review will briefly introduce the features of aptamer and potential of aptamer-based system for evaluation of vaccine efficacy.

Keyword

Aptamers; Vaccines; Enzyme-linked immunosorbent assay; Antigens; Antibodies

MeSH Terms

Antibodies
Enzyme-Linked Immunosorbent Assay
Vaccination
Vaccines
Antibodies
Vaccines

Figure

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  Fig. 1 Schematic cartoon for antibody (Ab) and aptamer and process of Ab and aptamer generation. (A) Structure of Ab and aptamer. (B) The process of Ab generation involves the following steps, such as mice vaccination, hybridoma generation and purification from culture supernatant of the hybridoma. (C) Aptamer is selected by systematic evolution of ligands by exponential enrichment process and amplification step in vitro.

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  Fig. 2 Immobilization process of aptamer on assay plate. Immobilization of aptamer on surface of assay plate using amine-moiety at the aptamer (A) or amine-moiety-containing nucleotide with complementary sequences to aptamer (B).

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