Int J Stem Cells.  2015 Nov;8(2):235-237. 10.15283/ijsc.2015.8.2.235.

Autologous Stem Cell Application in Periodontal Regeneration Technique (SAI-PRT) Using PDLSCs Directly From an Extracted Tooth...An Insight

• Vandana KL
• Desai R
• Dalvi PJ

• KMID: 2380818
• DOI: http://doi.org/10.15283/ijsc.2015.8.2.235

Abstract

Periodontal regeneration represents the ultimate goal of periodontal therapy. The current regenerative techniques have limited success rates especially in advanced periodontal defects. Currently the research is focused on novel cell-based approaches for periodontal regeneration to overcome the limitations of existing treatment. The human clinical trial on stem cells based periodontal regeneration is promising. The plethora of animal studies provide sound evidence to support the belief that periodontal ligament stem cells (PDLSCs) can be used for periodontal regeneration. The direct application of autologous periodontal stem cells in treatment of intrabony defects is attempted for the first time in periodontal literature. Stem cell Application in Periodontal Regeneration Technique (SAI-PRT) using direct PDLSCs has overcome the limitations and concerns of ex- vivo stem cell culture methods like high cost, technique sensitivity, loss of stemness during cell passage, genetic manipulation and tumorigenic potential. Clinical feasibility, success and cost effectiveness over currently available techniques are encouraging. The clinical utility of this novel idea is recommended.

Keyword

Periodontal ligament progenitors; Regeneration; Periodontitis

MeSH Terms

Animals
Cost-Benefit Analysis
Humans
Periodontal Ligament
Periodontitis
Regeneration*
Stem Cells*

Reference

References

1. Petersen PE, Ogawa H. Strengthening the prevention of periodontal disease: the WHO approach. J Periodontol. 2005; 76:2187–2193. DOI: 10.1902/jop.2005.76.12.2187. PMID: 16332229.

2. Agarwal V, Khatri M, Singh G, Gupta G, Marya CM, Kumar V. Prevalence of Periodontal Diseases in India. J Oral Health Comm Dent. 2010; 4(Suppl):7–16.

3. Young CS, Abukawa H, Asrican R, Ravens M, Troulis MJ, Kaban LB, Vacanti JP, Yelick PC. Tissue-engineered hybrid tooth and bone. Tissue Eng. 2005; 11:1599–1610. DOI: 10.1089/ten.2005.11.1599. PMID: 16259613.

4. Chen FM, Zhang J, Zhang M, An Y, Chen F, Wu ZF. A review on endogenous regenerative technology in periodontal regenerative medicine. Biomaterials. 2010; 31:7892–7927. DOI: 10.1016/j.biomaterials.2010.07.019. PMID: 20684986.

5. Nakahara T. A review of new developments in tissue engineering therapy for periodontitis. Dent Clin North Am. 2006; 50:265–276. DOI: 10.1016/j.cden.2005.11.004. PMID: 16530062.

6. Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY, Shi S. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004; 364:149–155. DOI: 10.1016/S0140-6736(04)16627-0. PMID: 15246727.

7. Wang L, Shen H, Zheng W, Tang L, Yang Z, Gao Y, Yang Q, Wang C, Duan Y, Jin Y. Characterization of stem cells from alveolar periodontal ligament. Tissue Eng Part A. 2011; 17:1015–1026. DOI: 10.1089/ten.tea.2010.0140.

8. Yan XZ, Yang F, Jansen JA, de Vries Rob BM, van den Beucken Jeroen JJP. Cell-based approaches in periodontal regeneration: a systematic review and meta-analysis of periodontal defect models in animal experimental work. Tissue Eng Part B Review. 2015; 21:411–426. DOI: 10.1089/ten.teb.2015.0049.

9. Lin NH, Gronthos S, Bartold PM. Stem cells and future periodontal regeneration. Periodontol 2000. 2009; 51:239–251. DOI: 10.1111/j.1600-0757.2009.00303.x. PMID: 19878478.

10. Chen FM, Sun HH, Lu H, Yu Q. Stem cell-delivery therapeutics for periodontal tissue regeneration. Biomaterials. 2012; 33:6320–6344. DOI: 10.1016/j.biomaterials.2012.05.048. PMID: 22695066.

11. Iwata T, Yamato M, Zhang Z, Mukobata S, Washio K, Ando T, Feijen J, Okano T, Ishikawa I. Validation of human periodontal ligament-derived cells as a reliable source for cytotherapeutic use. J Clin Periodontol. 2010; 37:1088–1099. DOI: 10.1111/j.1600-051X.2010.01597.x. PMID: 20618549.

12. Feng F, Akiyama K, Liu Y, Yamaza T, Wang TM, Chen JH, Wang BB, Huang GT, Wang S, Shi S. Utility of PDL progenitors for in vivo tissue regeneration: a report of 3 cases. Oral Dis. 2010; 16:20–28. DOI: 10.1111/j.1601-0825.2009.01593.x. PMID: 20355278. PMCID: 2848819.

13. Chen FM, Zhang M, Wu ZF. Toward delivery of multiple growth factors in tissue engineering. Biomaterials. 2010; 31:6279–6308. DOI: 10.1016/j.biomaterials.2010.04.053. PMID: 20493521.

14. Soto-Gutierrez A, Yagi H, Uygun BE, Navarro-Alvarez N, Uygun K, Kobayashi N, Yang YG, Yarmush ML. Cell delivery: from cell transplantation to organ engineering. Cell Transplant. 2010; 19:655–665. DOI: 10.3727/096368910X508753. PMID: 20525441. PMCID: 2957541.

15. Rohanizadeh R, Swain MV, Mason RS. Gelatin sponges (Gelfoam) as a scaffold for osteoblasts. J Mater Sci Mater Med. 2008; 19:1173–1182. DOI: 10.1007/s10856-007-3154-y.

16. Consensus report. Periodontal regeneration around natural teeth. Ann Periodontol. 1996; 1:667–670. DOI: 10.1902/annals.1996.1.1.667. PMID: 9118275.

17. Narayanan AS, Bartold PM. Biochemistry of periodontal connective tissues and their regeneration: a current perspective. Connect Tissue Res. 1996; 34:191–201. DOI: 10.3109/03008209609000698. PMID: 9023048.

18. Saygin NE, Giannobile WV, Somerman MJ. Molecular and cell biology of cementum. Periodontol 2000. 2000; 24:73–98. DOI: 10.1034/j.1600-0757.2000.2240105.x.

19. Zhu W, Liang M. Periodontal ligament stem cells: current status, concerns, and future prospects. Stem Cells Int. 2015; 2015:972313. DOI: 10.1155/2015/972313. PMID: 25861283. PMCID: 4378705.