Ann Dermatol.  2017 Jun;29(3):377-380. 10.5021/ad.2017.29.3.377.

Intense Pulsed Light Increases Hyaluronan and CD44 in Epidermal Keratinocytes and Improves Age-Related Epidermal Structure Defects in Mice

Affiliations
  • 1Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
  • 2Department of Applied Bioscience, CHA University, Seongnam, Korea.
  • 3Institute for Clinical Research, CHA University, Seongnam, Korea.
  • 4Department of Dermatology, CHA Bundang Medical Center, CHA University, Seongnam, Korea. derma97@gmail.com

Abstract

No abstract available.


MeSH Terms

Animals
Hyaluronic Acid*
Keratinocytes*
Mice*
Hyaluronic Acid

Figure

  • Fig. 1 Intense pulsed light (IPL) treatment increases hyaluronan (HA) production and hyaluronan synthases (HAS) 3 expression in young and old aged mouse epidermis and cultured human keratinocytes. (A) Sections of young and aged mouse skin treated with IPL were stained for HA binding protein (HABP) 4 and examined by confocal microscope. Dashed line is the dermal-epidermal junction. Magnified image shows example of accumulated HA in the intercellular (HAo) and intracellular space (HAi) (×400). After epidermal separation, HA content was measured by enzyme-linked immunosorbent assay (ELISA) (B) and the mRNA level of HABP4 (C) and HAS3 (D) was analyzed by quantitative real-time polymerase chain reaction (RT-PCR). Normal human epidermal keratinocytes (NHEK) were irradiated with IPL using either 510 nm or 570 nm cut-off filter. (E) ELISA for HA content and (F) quantitative RT-PCR analysis of HAS3 mRNA in conditioned medium from NHEK irradiated with IPL. Data represent the means±standard error. Data were analyzed by Student's t-test (*p<0.05, **p<0.01).

  • Fig. 2 Intense pulsed light (IPL) treatment increases CD44 expression in young and old aged mouse epidermis and cultured human keratinocytes and improves epidermal structure in aged mouse skin. (A) Immunohistochemical staining of CD44 in young and old aged mouse skin treated with IPL (×200). (B) After epidermal separation, CD44 mRNA level was analyzed by quantitative real-time polymerase chain reaction (RT-PCR). (C) CD44 mRNA level of conditioned medium from normal human epidermal keratinocytes (NHEK) irradiated with IPL using either 510 nm or 570 nm cut-off filter was analyzed by quantitative RT-PCR. Expression of differentiation marker, filaggrin (D) and proliferation marker, proliferating cell nuclear antigen (PCNA) (E) in aged mice skin treated with IPL was examined by immunohistochemical staining (×200). The stained area per epidermal area for filaggrin was measured (D) and cells with positive staining for PCNA per mm epidermal length were counted (E) by digital image analysis. Data represent the means±standard error (*p<0.05, **p<0.01).


Reference

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