Korean J Parasitol.  2015 Dec;53(6):789-793. 10.3347/kjp.2015.53.6.789.

Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)

Affiliations
  • 1Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China. liujingze@mail.hebtu.edu.cn
  • 2Shijiazhuang Post and Telecommunication Technical College, Shijiazhuang 050021, China.
  • 3Laboratory of Cell and Biochemistry, Institute of Biology, Hebei Academy of Sciences, Shijiazhuang, 050081, China.

Abstract

In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.

Keyword

Dermacentor silvarum; tick; antigenic protein; LC-MS/MS

MeSH Terms

Animals
Antigens/*chemistry/immunology
Arthropod Proteins/*chemistry/immunology
Electrophoresis, Polyacrylamide Gel
Female
Ixodidae/*chemistry/immunology
Molecular Weight
Rabbits
Tandem Mass Spectrometry
Antigens
Arthropod Proteins
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