Lab Anim Res.  2016 Sep;32(3):135-143. 10.5625/lar.2016.32.3.135.

Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination

Affiliations
  • 1Department of Biomedical Laboratory Science, Namseoul University, Cheonan, Korea. kang@nsu.ac.kr
  • 2Department of Health Administration, Namseoul University, Cheonan, Korea.

Abstract

The aim of this study was to examine the therapeutic potential of sulfasalazine and prednisolone in a mouse collagen antibody-induced arthritis (CAIA) model. Twenty-five male BALB/c mice were randomly divided into five groups: group 1 (G1): control, group 2 (G2): probe control, group 3 (G3): CAIA, group 4 (G4): CAIA+sulfasalazine (10 mg/kg, oral), and group 5 (G5): CAIA+prednisolone (100 mg/kg, oral). Fluorescence bioimaging was performed in vivo 24 and 48 h after treatment with a fluorescence probe (OsteoSense® 680 EX), and all mice were sacrificed. The hind knee joints were fixed in 10% neutral phosphate-buffered formalin, and micro-computed tomography (micro-CT) and histopathological analyses were performed. The paw thickness increased in a time-dependent manner in G3 mice, but trended toward a decrease in both G4 and G5 mice. Fluorescence intensity increased in G3 mice at 24 and 48 h after fluorescence probe treatment, but the fluorescence intensity in G4 and G5 mice was lower than that in G3. Micro-CT analyses showed that the joint surfaces of G3 mice had a rough and irregular articular appearance, but the occurrence of these irregularities was lower in G4 and G5. Hematoxylin and eosin and Safranin O-fast green staining confirmed that destruction of the cartilage and bony structures, synovial hyperplasia, and inflammatory cell infiltration all occurred in G3, and that the occurrence of these phenomena was lower in G4 and G5 than in G3. Taken together, these results suggest that sulfasalazine and prednisolone can reduce acute rheumatoid arthritis in mice.

Keyword

Mice; collagen antibody-induced arthritis; sulfasalazine; prednisolone fluorescence bioimaging; micro-computed tomography

MeSH Terms

Animals
Arthritis*
Arthritis, Rheumatoid
Cartilage
Collagen*
Eosine Yellowish-(YS)
Fluorescence
Formaldehyde
Hematoxylin
Humans
Hyperplasia
Joints
Knee Joint
Male
Mice*
Prednisolone
Sulfasalazine
Collagen
Eosine Yellowish-(YS)
Formaldehyde
Hematoxylin
Prednisolone
Sulfasalazine

Figure

  • Figure 1 Measurable morphological changes were determined by paw thickness measurement. (A) Macroscopic view of joint in mice. G1 as control, G2 as probe control, G3 as CAIA induced, G4 as CAIA+Sulfasalazine and G5 as CAIA+ Prednisolone. Note the normal macroscopic structure of the joint of mice in G1 and G2. However, in G3, the paw thickness was increased in mice treated with anti-type II collagen 5-clone antibody cocktail and it was decreased in G4 and G5 compared to G3.

  • Figure 2 In vivo near-infrared fluorescence (NIRF) bioimaging of knee joint of normal or collagen antibody-induced arthritis (CAIA) mice after intravenous injection of fluorescence probe. (A) G1 as control, G2 as probe control, G3 as CAIA induced, G4 as CAIA+Sulfasalazine, G5 as CAIA+Prednisolone; Fluorescence intensity in knee joint at 24 h (B) and 48 h (C) after fluorescence probe treatment. *,**Significant different from G1 (P<0.05 , P<0.01, respectively).

  • Figure 3 Micro-CT image of hind knee joint in mice. (A) G1 as control, (B) G2 as probe control, (C) G3 as CAIA induced, (D) G4 as CAIA + Sulfasalazine, (E) G5 as CAIA + Prednisolone. Note the joint surfaces of G1 or G2 as normal articular appearance. However, those of G3 showed rough and irregular articular appearance and it was decreased in G4 and G5 compared to G3 (arrow).

  • Figure 4 Micro-CT analysis parameters of the hind knee joint of collagen antibody-induced arthritis mice. (A) Bone volume; (B) Percent bone volume; (C) Bone surface/bone volume ratio; (D) Trabecular thickness; (E) Trabecular number; (F) Trabecular separation; (G) Bone mineral density; G1 as control, G2 as probe control, G3 as CAIA induced, G4 as CAIA+ Sulfasalazine and G5 as CAIA+Prednisolone. *Significantly different from G2 (P<0.05).

  • Figure 5 Histopathological examination of hind knee joint of mice. (A) G1 as control, (B) G2 as probe control, (C) G3 as CAIA induced, (D) G4 as CAIA+Sulfasalazine, (E) G5 as CAIA +Prednisolone. Note the normal microscopic structure of the joint of untreated control mice (A) and probe control mice (B). However, joint destruction is remarkable in the joint of mice treated with collagen antibody in G3. Note the destruction of cartilage and bony structure (arrow) and infiltration of inflammatory cells (arrow head) and these lesions were decreased in G4 and G5 compared to G3. Hematoxylin & eosin staining of paraffin embedded sections from the hind joint from mice, Magnification, ×200.

  • Figure 6 Safranin O-fast green staining of joint of mice. (A) G1 as control, (B) G2 as probe control, (C) G3 as CAIA induced, (D) G4 as CAIA+Sulfasalazine, (E) G5 as CAIA+ Prednisolone. Note the normal cartilage staining of the joint of mice in G1 and G2. In G3 cartilage staining is remarkably reduced in the joint of mice, however it shows moderate staining in G4 and G5. Safranin O-fast green staining of paraffin embedded sections from the hind joint from mice, Magnification, ×200.


Reference

1. Scott DL, Wolfe F, Huizinga TW. Rheumatoid arthritis. Lancet. 2010; 376(9746):1094–1108. PMID: 20870100.
Article
2. Khachigian LM. Collagen antibody-induced arthritis. Nat Protoc. 2006; 1(5):2512–2516. PMID: 17406499.
Article
3. Nandakumar KS, Svensson L, Holmdahl R. Collagen type II-specific monoclonal antibody-induced arthritis in mice: description of the disease and the influence of age, sex, and genes. Am J Pathol. 2003; 163(5):1827–1837. PMID: 14578183.
4. Hutamekalin P, Saito T, Yamaki K, Mizutani N, Brand DD, Waritani T, Terato K, Yoshino S. Collagen antibody-induced arthritis in mice: development of a new arthritogenic 5-clone cocktail of monoclonal anti-type II collagen antibodies. J Immunol Methods. 2009; 343(1):49–55. PMID: 19330909.
Article
5. de Jong M, Essers J, van Weerden WM. Imaging preclinical tumour models: improving translational power. Nat Rev Cancer. 2014; 14(7):481–493. PMID: 24943811.
Article
6. Chen Y, Liang CP, Liu Y, Fischer AH, Parwani AV, Pantanowitz L. Review of advanced imaging techniques. J Pathol Inform. 2012; 3:22. PMID: 22754737.
Article
7. Weissleder R, Tung CH, Mahmood U, Bogdanov A Jr. In vivo imaging of tumors with protease-activated near-infrared fluorescent probes. Nat Biotechnol. 1999; 17(4):375–378. PMID: 10207887.
Article
8. Lakey RL, Cawston TE. Sulfasalazine blocks the release of proteoglycan and collagen from cytokine stimulated cartilage and down-regulates metalloproteinases. Rheumatology (Oxford). 2009; 48(10):1208–1212. PMID: 19690126.
Article
9. Mihara K, Almansa C, Smeets RL, Loomans EE, Dulos J, Vink PM, Rooseboom M, Kreutzer H, Cavalcanti F, Boots AM, Nelissen RL. A potent and selective p38 inhibitor protects against bone damage in murine collagen-induced arthritis: a comparison with neutralization of mouse TNFalpha. Br J Pharmacol. 2008; 154(1):153–164. PMID: 18297096.
10. Bendele A. Animal models of rheumatoid arthritis. J Musculoskelet Neuronal Interact. 2001; 1(4):377–385. PMID: 15758488.
11. Knoerzer DB, Donovan MG, Schwartz BD, Mengle-Gaw LJ. Clinical and histological assessment of collagen-induced arthritis progression in the diabetes-resistant BB/Wor rat. Toxicol Pathol. 1997; 25(1):13–19. PMID: 9061845.
Article
12. Ying X, Monticello TM. Modern imaging technologies in toxicologic pathology: An overview. Toxicol Pathol. 2006; 34(7):815–826. PMID: 17178685.
Article
13. Peterson JD, Labranche TP, Vasquez KO, Kossodo S, Melton M, Rader R, Listello JT, Abrams MA, Misko TP. Optical tomographic imaging discriminates between disease-modifying anti-rheumatic drug (DMARD) and non-DMARD efficacy in collagen antibody-induced arthritis. Arthritis Res Ther. 2010; 12(3):R105. PMID: 20509880.
Article
14. Jain M, Shukla N, Manzoor M, Nadolny S, Mukherjee S. Modified full-field optical coherence tomography: A novel tool for rapid histology of tissues. J Pathol Inform. 2011; 2:28. PMID: 21773059.
Article
Full Text Links
  • LAR
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2023 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr