J Korean Ophthalmol Soc.  1987 Apr;28(2):279-284.

Cultured Corneal Keratocyte: Scanning and Transmission Electron Microscopic Findings

Affiliations
  • 1Department of Ophthalmology, School of Medicine, Hanyang University, Seoul, Korea.

Abstract

The cultured cells were derived from rabbit corneal stroma by explant technique following microdissection and serial passage. The ultrastructural features of fourteenth-passage keratocytes were examined with both scanning and transmission electron microscope. The cells can be divided into activated, intermediate and old cell according to the differences in electron density and surface microvillous pattern. The morphologic characteristics of the cultured keratocytes partially resemble those shown in corneal keratocytes in vivo.


MeSH Terms

Cells, Cultured
Corneal Keratocytes*
Corneal Stroma
Microdissection
Serial Passage
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