J Korean Rheum Assoc.  1994 Jul;1(2):200-212.

Immunoglobulin Gene Repertoire In Rheumatoid Synovium As A Model For Autoimmune Disease

• Lee SK
• Song JH

Abstract

OBJECTIVE
To gain insights into structural characteristics of immunoglobulin kappa chain repertoire expressed in the inflammed synovium of rheumatoid arthritis (RA), we analyzed V kappa transcirpts expressed in the synovium of a patient with longstanding RA and compared to those expressed in the PBLs of RA and normal controls.
METHODS
RT-PCR was done to amplify kappa chain transcripts from RA synovial lymphocytes and the cDNA sequences were compared to those from PBL of RA patient or normal control.
RESULTS
Kappa chain repertoire from RA patient's synovial lymphocytes or PBL revealed increased somatic mutation and unusually long complementarity determining region (CDR) 3 compared to normal control.
CONCLUSIONS
These changes in kappa chain repertoire in RA patient are suggesting that the antibody repertoire expressed in the synovium or PBL is unique and may be related with systemic dysregulation of B cell development

Keyword

Immunoglobulin gene; Repertoire; Rheumatoid arthritis; Somatic mutation; CDR 3 lengths

MeSH Terms

Arthritis, Rheumatoid
Autoimmune Diseases*
Complementarity Determining Regions
DNA, Complementary
Genes, Immunoglobulin*
Humans
Immunoglobulin kappa-Chains
Immunoglobulins*
Lymphocytes
Synovial Membrane*
Complementarity Determining Regions
DNA, Complementary
Immunoglobulin kappa-Chains
Immunoglobulins