J Korean Acad Prosthodont.  2014 Oct;52(4):298-304. 10.4047/jkap.2014.52.4.298.

Osseointegration of the titanium implant coated with rhTGF-beta2/PLGA particles by electrospray: a preliminary microCT analyzing rabbit study

• Lee WS
• Kim SK
• Heo SJ
• Koak JY
• Lee JH
• Park JM
• Park YK

• KMID: 2321791
• DOI: http://doi.org/10.4047/jkap.2014.52.4.298

Abstract

PURPOSE
This preliminary rabbit study was conducted to evaluate the effect of recombinant human transforming growth factor-beta2 (rhTGF-beta2)/poly lactic-co-glycolic acid (PLGA) coating on osseointegration of the titanium (Ti) implant.
MATERIALS AND METHODS
Eight Ti implants were anodized with 300 voltages for three minutes. Four of those were coated with rhTGF-beta2/PLGA by an electrospray method as the experimental group. The implants were placed into tibiae of four New Zealand rabbits, two implants per a tibia, one implant per each group. After 3 and 6 weeks, every two rabbits were sacrificed and micro-computed tomography (microCT) was taken for histomorphometric analysis.
RESULTS
In scanning electron microscope (SEM) image, the surface of rhTGF-beta2/PLGA coated Ti implant showed well distributed particles. Although statistically insignificant, microCT analysis showed that experimental group has higher bone volume / total volume (BV/TV) and trabecular thickness (Tb.Th) values relatively. Cross sectional view also showed more newly formed bone in the experimental group.
CONCLUSION
In the limitation of this study, rhTGF-beta2/PLGA particles coating on the Ti implant show the possibility of more favorable quantity of newly formed bone after implant installation.

Keyword

TGF-beta2; PLGA; Titanium; Implant; Osseointegration

MeSH Terms

Humans
Osseointegration*
Rabbits
Tibia
Titanium*
Transforming Growth Factor beta2
X-Ray Microtomography*
Titanium
Transforming Growth Factor beta2

Figure

• 

  Fig. 1. Schematic image of implant. (A) Region of interest (ROI) for microCT,(B) reference for cross sectional view.

• 

  Fig. 2. SEM image of implant surface (×5,000). (A) Control group (implant without surface coating), (B) Experimental group (implant with rhTGF-β 2/PLGA coating via electrospray method).

• 

  Fig. 3. MicroCT analysis. (A) BV/TV (bone volume per total volume), (B) SMI (structure model index), (C) Tb.Th (trabecular thickness), (D) Tb.N (trabecular number), C (control), E (experimental) (P>.05).

• 

  Fig. 4. Cross sectional view of microCT. (A) 3 weeks control, (B) 3 weeks experimental (rhTGF-β 2/ PLGA coated implant), (C) 6 weeks control, (D) 6 weeks experimental (rhTGF-β 2/ PLGA coated implant).

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