Nutr Res Pract.  2015 Feb;9(1):43-48. 10.4162/nrp.2015.9.1.43.

Regular moderate exercise training can alter the urinary excretion of thiamin and riboflavin

  • 1Department of Food & Nutrition, Duksung Women's University, 33, Samyangro 114 gil, Dobong-gu, Seoul, 132-714, Korea.


Physical exercise promotes energy producing pathways requiring thiamin and riboflavin as a coenzyme. Therefore, this study investigated the effects of regular exercise training on urinary excretion of thiamin and riboflavin.
Fifty rats were randomly assigned to one of two groups: non-exercise training (NT, n = 25) and regular exercise training (ET, n = 25) for 5 weeks. The rats performed moderate exercise on a treadmill (0.5-0.8 km/hour) for 30 min/day, 5 days/week. Twenty-four hour urine samples were collected at the end of the 0 week, 3rd week, and 5th week of training and thiamin and riboflavin were analyzed.
No significant differences in thiamin and riboflavin intakes for each week were observed between the NT and ET groups. Urinary thiamin excretion of each group was the highest at the 5th week compared to the levels at 0 and 3rd week. Urinary thiamin at the 5th week was significantly lower in the ET group than in the NT group. Urinary riboflavin excretion was increased by training duration, however, no difference was observed between NT and ET for each week. At 0 and 3rd week, no significant relationships were observed between dietary intake and urinary excretion of thiamin and riboflavin, however, at the 5th week, urinary excretion was significantly increased by dietary intake only in the NT group (P < 0.05). Thiamin excretion of both NT and ET groups was significantly increased with riboflavin excretion at the 5th week (P < 0.01).
Regular moderate exercise training increased urinary excretion of thiamin. Dietary intakes and urinary excretions of thiamin and riboflavin showed positive correlation in both the exercise training and non-exercise training groups as the exercise training period went by, while the correlations in the exercise training group were weaker than those in the non-exercise training group. Therefore, regular exercise training can alter the urinary excretion of thiamin and riboflavin in rats.


Thiamin; riboflavin; exercise training; B vitamins; rats

MeSH Terms

Vitamin B Complex
Vitamin B Complex


  • Fig. 1 The effect of regular exercise training on the urinary excretion of thiamin. abcA different superscript letter indicates significant difference at P < 0.05 in each group by a duration of regular exercise training. * Urinary thiamin levels were significant in the non-exercise training and exercise training groups at P < 0.05.

  • Fig. 2 The effect of regular exercise training on the urinary excretion of riboflavin. abcA different superscript letter indicates significant difference at P < 0.05 in each group by duration of regular exercise training. There were no significant differences of urine riboflavin between groups at 0, 3rd, and 5th week.

  • Fig. 3 The effect of regular exercise training on the relations between the intake and urinary excretion of thiamin

  • Fig. 4 The effect of regular exercise training on the relations between the intake and urinary excretion of riboflavin

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