Nutr Res Pract.  2014 Oct;8(5):550-557. 10.4162/nrp.2014.8.5.550.

Bioavailability of plant pigment phytochemicals in Angelica keiskei in older adults: A pilot absorption kinetic study

• Correa CR
• Chen CY
• Aldini G
• Rasmussen
• Ronchi CF
• Berchieri-Ronchi C
• Cho SM
• Blumberg J
• Yeum KJ

• KMID: 2313778
• DOI: http://doi.org/10.4162/nrp.2014.8.5.550

Abstract

BACKGROUND/OBJECTIVES
Angelica keiskei is a green leafy vegetable rich in plant pigment phytochemicals such as flavonoids and carotenoids. This study examined bioavailability of flavonoids and carotenoids in Angelica keiskei and the alteration of the antioxidant performance in vivo. SUBJECTS AND MATERIALS: Absorption kinetics of phytochemicals in Angelica keiskei were determined in healthy older adults (> 60 y, n = 5) and subjects with metabolic syndrome (n = 5). Subjects consumed 5 g dry Angelica keiskei powder encapsulated in gelatin capsules with a low flavonoid and carotenoid liquid meal. Plasma samples were collected at baseline, 0.5, 1, 2, 3, 4, 5, 6, 7, and 8 h. Samples were analyzed for flavonoids and carotenoids using HPLC systems with electrochemical and UV detection, respectively, and for total antioxidant performance by fluorometry.
RESULTS
After ingestion of Angelica keiskei increases in plasma quercetin concentrations were observed at 1-3 and 6-8 hr in the healthy group and at all time points in the metabolic syndrome group compared to baseline (P < 0.05). Plasma lutein concentrations were significantly elevated in both the healthy and metabolic syndrome groups at 8 hr (P < 0.05). Significant increases in total antioxidant performance were also observed in both the healthy and the metabolic syndrome groups compared to baseline (P < 0.05).
CONCLUSIONS
Findings of this study clearly demonstrate the bioavailability of phytonutrients of Angelica keiskei and their ability to increase antioxidant status in humans.

Keyword

Angelica keiskei; quercetin; lutein; absorption kinetic; total antioxidant performance

MeSH Terms

Absorption*
Adult*
Angelica*
Biological Availability*
Capsules
Carotenoids
Chromatography, High Pressure Liquid
Eating
Flavonoids
Fluorometry
Gelatin
Humans
Kinetics
Lutein
Meals
Phytochemicals*
Plants*
Plasma
Quercetin
Vegetables
Capsules
Carotenoids
Flavonoids
Gelatin
Lutein
Quercetin

Figure

• 

  Fig. 1 Absorption kinetics of quercetin in plasma after ingesting 5 g of Angelica keiskei in older adults (aged ≥ 60 yr); healthy (A) and metabolic syndrome (B). Data are expressed as mean ± SEM (n = 5 in each group). ND, not detected. One-way repeated measures ANOVA with Tukey's multiple comparison was performed. When an equal variance test failed, Friedman repeated measures analysis of variance on ranks with Tukey's multiple comparison test was used. Significantly different as compared with the baseline *P < 0.05. Samples were analyzed by HPLC with ECD detection for quercetin.

• 

  Fig. 2 Absorption kinetics of lutein in plasma after ingesting 5 g of Angelica keiskei in older adults (aged ≥ 60 yr); healthy (A) and metabolic syndrome (B). Data are expressed as mean ± SEM (n = 5 in each group). One-way repeated measures ANOVA with Tukey's multiple comparison was performed. Significantly different as compared with the baseline *P < 0.05. Samples were analyzed by HPLC with UV detection.

• 

  Fig. 3 Plasma total antioxidant performance after ingesting 5 g of Angelica keiskei in older adults (aged ≥ 60 yr); healthy (A) and metabolic syndrome (B). Data are expressed as mean ± SEM (n = 5 in each group). One-way repeated measures ANOVA with Tukey's multiple comparison was performed. When an equal variance test failed, Friedman repeated measures analysis of variance on ranks with Tukey's multiple comparison test was used. Significantly different as compared with the baseline *P < 0.05. Samples were analyzed by fluorometry.

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