Mycobiology.  2007 Dec;35(4):226-229.

The Artificial Cultivation of Oudemansiella mucida on the Oak Sawdust Medium

• Lee GW
• Jaysinghe
• Imtiaj A
• Shim MJ
• Hur H
• Lee MW
• Lee KR
• Kim SH
• Kim HY
• Lee UY
• Lee TS

Abstract

To produce fruiting bodies of Oudemansiella mucida, porcelain fungus, on the oak sawdust medium, additives suitable for the mycelial growth and fruiting body formation were screened. In general, the mycelial growth of the three strains of O. mucida used in this study have been good on oak sawdust mixed rice bran of 20~30%. The mycelia incubated in potato dextrose broth for 7 days were inoculated on oak sawdust medium supplemented with various ratios of rice bran and incubated for 30 days at 25degrees C in the dark condition until the mycelia of O. mucida fully colonized the media from top to bottom. Then, top surface of the media in the bottles were horizontally scratched with a spatula and filled with tap water for 3 hours. To induce the primordial formation of O. mucida, the bottles were transferred to the mushroom cultivating room under 12 hrs of light (350 lux) and dark condition with relative humidity of 95% at 17degrees C. The primordia of O. mucida were formed on the surface of oak sawdust media after 7 days of incubation. The mature fruiting bodies were observed 5 days after primordial formation. The fruiting bodies O. mucida were formed on oak sawdust medium mixed with 5 to 30% rice bran. However, abundant fruiting-bodies of O. mucida were produced in oak sawdust medium supplemented with 20% rice bran. This is the first report associated with an artificial fruiting body production of O. mucida in Korea.

Keyword

Additives; Artificial cultivation; Fruiting bodies; Oudemansiella mucida; Sawdust medium

MeSH Terms

Agaricales
Colon
Dental Porcelain
Fruit
Fungi
Glucose
Humidity
Korea
Solanum tuberosum
Water
Dental Porcelain
Glucose
Water