Lab Med Online.  2012 Jul;2(3):139-147.

Analysis of Polycheck Allergy Results of the Recent Two Years: Comparison with Skin Prick Test and ImmunoCAP

Affiliations
  • 1Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea. jhyoo92@empal.com
  • 2Department of Pediatrics, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea.
  • 3Department of Dermatology, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea.
  • 4Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.
  • 5Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA.

Abstract

BACKGROUND
Multiple Antigen Simultaneous Test (MAST)-immunoblot assay is a practical and economical test, which has been recently introduced nationwide. Authors investigated test efficiency of a MAST-immunoblot assay, Polycheck Allergy (PA).
METHODS
A total of 3,153 patients were tested by PA and the results were compared with the results of ImmunoCAP and skin prick test (SPT) in 532 and 75 patients, respectively. The correlation with the lgE results measured by VIDAS was also analyzed.
RESULTS
The agreements of PA with SPT were 87.8% in the Inhalant Panel and 89.3% in the Food Panel and the agreement of ImmunoCAP with SPT was 95.2%. The most common allergens giving positive reactions were Dermatophagoides farinae (46.2%) and Dermatophagoides pteronyssinus (40.0%). SPT taken as a reference, PA compared with ImmunoCAP showed higher agreement (D. farinae, 76.0 vs. 70.7%; D. pteronyssinus, 76.0 vs. 74.4%), sensitivity (D. farinae, 72.7 vs. 68.2%; D. pteronyssinus, 75.0 vs. 71.2%) and specificity (D. farinae, 85.0 vs. 81.3%) except for the specificity for D. pteronyssinus (78.3 vs. 87.5%). The rate of allergen specific IgE positive patients was higher than that of negative patients when total IgE was over 100 kU/L.
CONCLUSIONS
Our results showed that the agreement, sensitivity and specificity of PA were similar to or better than those of the previously established test, ImmunoCAP. The allergen specific IgE results of PA were in correlation with total IgE. PA may be used for testing allergen specific IgE to diagnose and treat allergic diseases.

Keyword

Allergy; Polycheck Allergy; ImmunoCAP; Skin prick test; Allergen-specific IgE
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