Korean J Dermatol.
2006 Jan;44(1):22-27.
Analysis of Hair Protein from Extrinsic Hair Damage
- Affiliations
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- 1Department of Dermatology and Institute of Hair & Cosmetic Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea. leews@wonju.yonsei.ac.kr
Abstract
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BACKGROUND: Various kinds of hair products are widely used due to the increase of interest in hair styling. Cosmetic procedures such as permanent waving are very popular today, but the medical studies related to the meaning and restoration pattern of hair damage are mainly based on structural findings. In measuring the degree of hair damage by the moleculobiological methods rather than the structural studies, the findings seem to be highly objective and standardized.
OBJECTIVE
The purpose of this study was to identify the patterns of hair damage and restoration through electrophoresis and western blot analysis of hair proteins.
METHODS
The three volunteers who we selected as subjects did not have any specific medical illness and had not performed any special cosmetic procedures which could have caused hair damage during the six months before the study. We conducted permanent waving on them. Human hair samples were obtained from the occipital scalp, which were that was not affected by the androgen. We performed extraction and concentration of the whole and partial hair protein, then operated electrophoresis and western blot analysis of the hair protein.
RESULTS
In the western blot analysis of whole hair proteins, there was one positive finding on subject A. This may have resulted from the small amount of partial proteins among the whole hair proteins. In the western blot analysis of partial hair proteins, subject A and B showed positive findings. In particular, positive findings were found on the 14th week of the experiment.
CONCLUSION
These results show a change in the hair proteins due to hair damage, and ultrastructurally, we found the possibility of prologation of actual hair damage longer than expected.
