Infect Chemother.
2004 Aug;36(4):241-244.
Immunoreactivity of Iron-Repressible Cell Wall Proteins and Exoproteins of Staphylococcus aureus
- Affiliations
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- 1Department of Microbiology, Chosun University Medical School, Kwangju, Korea. shsin@chosun.ac.kr
- 2Department of Internal Medicine, Chosun University Medical School, Kwangju, Korea.
- 3Department of Emergency Medicine, Chosun University Medical School, Kwangju, Korea.
- 4Research Center for Resistant Cells, Chosun University Medical School, Kwangju, Korea.
Abstract
- In the present study, we tried to investigate immunoreactivity of iron-repressible cell wall proteins and exoproteins of Staphylococcus aureus when the convalescent serum obtained from the patient with septicemia was applied. On SDS-PAGE and western blot analysis, several iron-repressible cell wall proteins expressed in iron-deficient BHI showed strong immunoreactivity, whereas no or relatively weak immunoreactivity was shown in iron-sufficient BHI. Several exoproteins were expressed only in iron-deficient BHI and these exoproteins showed strong immunoreactivity. These results indicate that several iron-repressible cell wall proteins and exoproteins are expressed and are immunogenic in vivo. Since in vivo state is an relatively iron-restricted condition, we recommend the use of iron-deficient media for studies concerning pathogenicity of staphylococcal in human.
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