Korean J Phys Anthropol.  2004 Jun;17(2):131-137.

Distribution of von Willebrand Factor with Angiogenesis in Neonatal Rat Heart

• Lee BH
• Paik DJ
• Kim WK
• Chung HS

Abstract

To investigate the process of angiogenesis in the cardiac tissue of neonatal rats, the levels of von Willebrand factor (vWf) produced by endothelial cells were observed. At days 1 to 7 after birth, whole heart obtained from neonatal rats was frozen sectioned, stained with anti-vWf and biotinylated rabbit anti-goat IgG antibodies, followed by immunohistochemical examinations. The results were as follows: 1. At day 1 after birth, extracellular matrix of endocardium and epicardium was stained with anti-vWf at the intermediate level, but that of myocardium was at the low level. 2. At day 2 after birth, a few blood islands were detected. At day 4 after birth, blood island was formed in most parts of heart and extracellular matrix was stained with ant-vWf at the intermediate level. 3. At day 7 after birth, a few blood vessels were formed, and endothelial cells and extracellular matrix was stained with ant-vWf at the intermediate level. These results suggest that mesenchymal cells were differentiated to blood islands and myocardiac cells, which are responsible for the distribution of vWf in extracellular matirx and for angiogenesis.

Keyword

Neonate of rat; Angiogenesis; von Willebrand factor; Heart

MeSH Terms

Animals
Antibodies
Blood Vessels
Endocardium
Endothelial Cells
Extracellular Matrix
Heart*
Immunoglobulin G
Islands
Myocardium
Parturition
Pericardium
Rats*
von Willebrand Factor*
Antibodies
Immunoglobulin G
von Willebrand Factor