Ann Dermatol.  2012 Nov;24(4):444-452. 10.5021/ad.2012.24.4.444.

Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5

• Park HJ
• Lee YW
• Choe YB
• Ahn KJ

• KMID: 2266040
• DOI: http://doi.org/10.5021/ad.2012.24.4.444

Abstract

BACKGROUND
Skin pigmentary changes of pityriasis versicolor may occur as either hyperpigmented or hypopigmented lesions, depending on the outcome of interactions between Malassezia yeasts and the skin, such as lipoperoxidation process, stimulus of inflammatory cell to melanocytes, and increased thickness of keratin layer.
OBJECTIVE
To investigate skin characteristic factors that enhance the susceptibility to Malassezia yeasts and provoke different color changes of pityriasis versicolor patients.
METHODS
To clarify these factors, we investigated the skin characteristics of pityriasis versicolor patients, using a non-invasive method known as MPA 5(R) (Courage and Khazaka, Germany). A total of 90 normal healthy subjects and 30 pityriasis versicolor patients were included in this study.
RESULTS
Both hyperpigmented and hypopigmented pityriasis versicolor skin lesions showed higher humidity, increased sebum excretion rate and increased transepidermal water loss (TEWL) values than normal healthy subjects. But no significant difference of specific Malassezia yeasts species between hyperpigmented and hypopigmented skin lesions was evident.
CONCLUSION
These results indicate that higher humidity and increased sebum level provide a better growing environment of Malassezia yeasts in the skin, leading to the assumption that interaction between Malassezia yeasts and skin barrier materials makes disruption of skin barrier causing increased TEWL.

Keyword

Malassezia yeasts; MPA 5 (Multiprobe Adapter 5); Pityriasis versicolor

MeSH Terms

Humans
Humidity
Keratins
Malassezia
Melanocytes
Pityriasis
Sebum
Skin
Tinea Versicolor
Water Loss, Insensible
Yeasts
Keratins
Water Loss, Insensible

Figure

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  Fig. 1 (A, C) Hyperpigmented lesions of two representative patients on the back and anterior chest. (B, D) Hypopigmented lesions of two representative patients on the cheek and posterior neck.

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  Fig. 2 (A) Comparison of TEWL values, (B) sebum excretion rate, (C) capacitance values, (D) melanin index and (E) erythema index between healthy control and patients. TEWL: transepidermal water loss, M: male, F: female. t-test paired comparison were performed versus healthy control; the *shows statistically significant differences at p<0.05.

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  Fig. 3 PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

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  Fig. 4 PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker, 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

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