Korean J Blood Transfus.  2015 Aug;26(2):123-131. 10.17945/kjbt.2015.26.2.123.

Correlation between the Severity of Bone Pain and the Amount of CD34+ Cells after Peripheral Blood Stem Cell Mobilization

• Seo JW
• Choi JS
• Kim YH
• Yoon SS
• Park S
• Han KS

• KMID: 2255808
• DOI: http://doi.org/10.17945/kjbt.2015.26.2.123

Abstract

BACKGROUND
Peripheral blood stem cells (PBSCs) are mobilized by granulocyte-colony stimulating factor (G-CSF), which causes several side effects in allogeneic donors. We report on side effects of G-CSF administration and determine which side effects could be used in predicting the amount of harvested CD34+ cells.
METHODS
Data from the first PBSC collections of 155 healthy donors between 2007 and 2010 were analyzed. Side effects were assessed using adverse event inventory, which was graded from 1 (mild) to 3 (severe) or 4 (disabling).
RESULTS
G-CSF administration caused an elevation of WBC counts (mean 44,834/microL) and 86% of them were neutrophils. The mean mononuclear cells in apheresis products was 6.6x10(8)/kg and mean CD34+ cells was 6.0x10(6)/kg. Bone pain was reported by 151 healthy donors (97%) and severe bone pain was related to more CD34+ cells in apheresis products (P=0.041): 39 for grade 1 (5.1x10(6) CD34+cells/kg), 86 for grade 2 (6.0x10(6)), and 26 for grade 3 (7.1x10(6)). In addition, the percentage of collecting more than 5.0x10(6) CD34+cells/kg during the first leukapheresis showed correlation with the severity of bone pain.
CONCLUSION
Bone pain was the most common side effect of G-CSF mobilization and more CD34+ cells were harvested in cases of severe bone pain.

Keyword

Peripheral blood stem cell; Bone pain; G-CSF; CD34 positive cell; Side effect

MeSH Terms

Blood Component Removal
Granulocyte Colony-Stimulating Factor
Hematopoietic Stem Cell Mobilization*
Humans
Leukapheresis
Neutrophils
Stem Cells*
Tissue Donors
Granulocyte Colony-Stimulating Factor