Korean J Nephrol.  2006 Mar;25(2):229-234.

Urinary Transforming Growth Factor-beta-inducible Gene-h3 in Patients with Glomerular Diseases

• Hong HJ
• Kim SD
• Lee BC
• Yoon HJ
• Bae EH
• Cho BS

Abstract

BACKGOUND: Transforming growth factor-beta (TGF-alpha) has been implicated in the pathogenesis of a number of kidney diseases. However, TGF-alpha is secreted in a latent form requiring extracellular modification to become biologically active. Recently, the activity of TGF-alpha has been assessed by the measurement betaig-h3, a novel TGF-alpha induced gene product. Thus we evaluated the pattern of urinary betaig-h3 expression in various glomerular diseases.
METHODS
64 patients with biopsy-proven primary glomerulonephritis (FSGS 6, HSPN 16, IgAN 20, MPGN I 7, and MesPGN 15), 10 patients with nephrotic syndrome and 12 healthy controls were enrolled in the study. A total 86 subjects (51 males, 59.3% and 35 females, 40.7%, mean age 13.9+/-4.28 years) constituted study population. First morning urine were collected and betaig-h3 in the urine was determined by indirect competitive ELISA (Regen Biotech Inc, Seoul, Korea).
RESULTS
betaig-h3 excretion was significantly higher in the urine from patients with HSPN (27.5+/-6.46, p=0.002), with IgAN (40.83+/-12.27, p=0.026), with MPGN I (21.64+/-7.29, p=0.042), MesPGN (26.42+/-6.68, p=0.007). In patients with FSGS (21.65+/-17.12) and minimal change nephrotic syndrome (6.26+/-2.18), mean urinary betaig-h3 excretion was not significant higher than that in control group (3.56+/-0.78).
CONCLUSION
Urinary betaig-h3 excretion was high in proliferative renal diseases. However, betaig-h3 excretion was not high in non-proliferative renal diseases.

MeSH Terms

Enzyme-Linked Immunosorbent Assay
Female
Glomerulonephritis
Glomerulonephritis, Membranoproliferative
Humans
Kidney Diseases
Male
Nephrosis, Lipoid
Nephrotic Syndrome
Seoul
Transforming Growth Factor alpha
Transforming Growth Factor alpha