Korean J Dermatol.  1975 Sep;13(3):159-165.

Inhibitory Effects of Some Antifungal Agents on Germination Activities of Conidiospores

• Kim MY
• Kim HS

Abstract

We studied germination activities of fungus conidiospores in active growth phase using the hanging slide culture method, then investigated the inhibitory effects of two antifungal agents(Tolnaftate and Clotrimazole) on germination activities. The fungal strains used in this study were Trichophyton (T.) rubrum, T. mentagrophytes and Candida (C.) albicans. Each strain was cultured on the Sabouraud's glueose agar plate and examined daily for detection of active growth phase of conidiospores (blastospores in C. albicans). After the conidiospores reached to active growth phase the activated spores were inoculated in one drop of Sabouraud's glucose broth on the cover glass and the hanging slide cultures were performed. The mean hermination rates of T. rubrum and T. nentagrophytes were 87.5% and 78% respectively in 72 hours. In C.albicans the average germination rate was 85.7% after cultivation for 24 hours, this germination rate was much faster than that of T.rubrum and T.mentagrophytes. For detection of miniman inhibitory concentration (MIC) of the antifungal agents to germination activities the antifungal agents were diluted with Sabouraud's glucose broth to various concentration and hanging slide culture was performed with the media containing the antifungal remedies. The minimal concentration of antifungal agents in which the conidiospores could completely not be germinated was denoted to minimal inhibitory concentration. The MIC of Tolnaftate to T.rubrum and T.mentagrophytes were 0.0.1ug/ml. The MIC of Cloirimazole to T. rubrum and T.mentagrophytes were 0.1ug/ml and to C. albicans was 1ug/ml. This results were similar to the previous reports by other investigators. This is a new method that could be applicated for evaluation of antifungal activities of antifungal agents.

MeSH Terms

Agar
Antifungal Agents*
Candida
Fungi
Germination*
Glass
Glucose
Humans
Research Personnel
Spores
Tolnaftate
Trichophyton
Agar
Antifungal Agents
Glucose
Tolnaftate