Korean J Anat.  2004 Jun;37(3):275-281.

Fyn Tyrosine Kinase-mediated Tyrosine Phosphorylation of Roundabout (Robo), the Slit Receptor

Affiliations
  • 1Department of Anatomy, Inje University Medical School, Korea. phwantae@daunet.donga.ac.kr
  • 2Department of Physiology and Institute of Medical Science, College of Medicine, Dong-A University, Korea.

Abstract

In this study, the molecular mechanism of tyrosine phosphorylation of Roundabout (Robo), the transmembrane receptor for slits, was investigated. The tyrosine phosphorylation of intracellular portion of Robo was increased by the treatment of tyrosine phosphatase inhibitors in human embryonic kidney cells transfected with Robo. The Robo tyrosine phosphorylation was inhibited by the treatment of Src family kinase inhibitor, PP2. The co-transfection of constitutively active form of Fyn, not the dominant negative form of Fyn, and Robo dramatically enhanced the tyrosine phosphorylation of Robo. Furthermore, the SH2 domain of Fyn, which binds to phosphorylated tyrosine residues, interact with Robo, and the interaction was increased by the inhibition of tyrosine phosphatases. These findings indicate that the tyrosine phosphorylation of Robo is regulated by Fyn.

Keyword

Robo; Slit; Axon guidance; Neuronal migration; Tyrosine phosphorylation; Fyn
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