Korean J Physiol Pharmacol.  2004 Apr;8(2):111-115.

Multiple Regulation of Roundabout (Robo) Phosphorylation in a Heterologous Cell System

Affiliations
  • 1Department of Physiology and Institute of Medical Science, College of Medicine, Dong-A University, Busan 602-714, Korea.

Abstract

Roundabout (Robo) is the transmembrane receptor for slit, the neuronal guidance molecule. In this study, the tyrosine phosphorylation of Robo was observed in Robo-transfected human embryonic kidney cells and developing rat brains, and found to be increased by the treatment with protein kinase A activator, forskolin. In contrast, protein kinase C activation by phorbol-12-myristate-13-acetate decreased the phosphorylation of Robo. Intracellular calcium was required for the tyrosine phosphorylation. Furthermore, the transfection of an Eph receptor tyrosine kinase dramatically enhanced the tyrosine phosphorylation. These findings indicate that the tyrosine phosphorylation of Robo is regulated by multiple mechanisms, and that Eph receptor kinases may play a role in the regulation of tyrosine phosphorylation of Robo in the rat brain.

Keyword

Robo; Slit; Axon guidance; Protein kinases; Tyrosine phosphorylation
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