J Korean Ophthalmol Soc.  2014 Sep;55(9):1366-1371. 10.3341/jkos.2014.55.9.1366.

The Effects of Commodified Growth Factor Products on the Fibrovascularization of Porous Polyethylene Orbital Implants

• Gu WM
• Choi JH
• Son JH

• KMID: 2217122
• DOI: http://doi.org/10.3341/jkos.2014.55.9.1366

Abstract

PURPOSE
To investigate the effects of commodified growth factor products used clinically on fibrovascular ingrowth into porous polyethylene orbital implants.
METHODS
Porous polyethylene orbital implant sheets (Medpor(R)) soaked with Nepidermin (Easyef(R)), Trafermin (Fiblast(R)), and normal saline were implanted into the backs of 18 Sprague-Dawley rats. The degree of fibrovascular ingrowth as observed using a light microscope was compared 1 and 2 weeks after implantation and was calculated as a percentage of the fibrovascular ingrowth length.
RESULTS
One week after implantation, the percentage of fibrovascular ingrowth length was 25.33 +/- 5.43%, 22.56 +/- 5.30%, and 21.78 +/- 4.66% in the Easyef(R)-, Fiblast(R)- and normal saline-soaked groups. The degree of fibrovascularization was higher in the Easyef(R)-soaked group than in the other groups (p = 0.020, 0.012). Two weeks after implantation, the degree of fibrovascularization was 98.33 +/- 5.00%, 100.00 +/- 0.00%, and 95.89 +/- 4.57%, which was significantly higher in the Easyef(R)-, and Fiblast(R)-soaked groups than in normal saline-soaked group (p = 0.019, <0.001).
CONCLUSIONS
Commodified growth factor products used in other areas selectively enhanced fibrovascular ingrowth to a greater degree and earlier in ophthalmic plastic and reconstructive surgery.

Keyword

Basic fibroblast growth factor; Epidermal growth factor; Fibrovascularization; Porous polyethylene orbital implant

MeSH Terms

Epidermal Growth Factor
Fibroblast Growth Factor 2
Orbital Implants*
Plastics
Polyethylene*
Rats, Sprague-Dawley
Epidermal Growth Factor
Fibroblast Growth Factor 2
Plastics
Polyethylene

Figure

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  Figure 1. Porous polyethylene sheets soaked with Easyef® (Nepidermin, Group E), Fiblast® (Trafermin, Group F), normal saline (Group N).

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  Figure 2. Postoperative photograph of the mice. Medpor® sheets soaked with Easyef® (Nepidermin, Group E), Fiblast® (Trafermin, Group F), normal saline (Group N) were implanted into mice back. Each group was implanted on each both sides in the upper, middle, and lower part, respectively.

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  Figure 3. Schematic diagram depicting the fibrovascular ingrowth of each sheet central plane. (A) 3 mm thickness Medpor® sheet cut into 1 mm width in the central plane of each sheet (broken line). (B) Fibrovascular ingrowth length was examined in 2 directions (a, b) and measured at 2 points. Each fibrovascular ingrowth length (%) = (a + b)/3 × 100. The degree of fibrovascularization was calculated an average of the each fibrovascular ingrowth length.

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  Figure 4. Determination of fibrovascular ingrowth margin in hematoxylin & eosin staining sheet under a light microscope. This sheet is Easyef® (Nepidermin) soaked sheet at 1 week after implantation. The fibrovascular ingrowth margin was drawn with a dotted line. (A) Low magnification view (×10) of cross section of the sheet. (B) Higher magnification view (×40) of the box area in (A). A connective tissue enmeshed with spindle-shaped fibroblasts can be seen on the lower side of the picture.

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  Figure 5. Hematoxylin & eosin staining (×10) of extracted porous polyethylene sheets showing that Easyef® (Nepidermin)-, Fiblast® (Trafermin)-soaked sheets have more fibrovascularization than normal saline-soaked sheet 2 weeks after implantation. (A) Soaking of sheet in Easyef® (Nepidermin). (B) Soaking of sheet in Fiblast® (Trafermin). (C) Soaking of sheet in normal saline.

Reference

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