J Korean Ophthalmol Soc.  2013 Apr;54(4):639-644. 10.3341/jkos.2013.54.4.639.

Effect of Nitric Oxide on Adhesion and Migration of Trabecular Meshwork Cells

Affiliations
  • 1Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr

Abstract

PURPOSE
To investigate the effect of nitric oxide (NO) on the adhesion and migration of cultured human trabecular meshwork cells (HTMC).
METHODS
For adhesion assay, primarily cultured HTMC were attached to culture dishes for 1 hr, cells were rinsed, and the remaining adherent cells were assessed with MTT assay. Degree of cellular migration was assessed under normal and stressed conditions using microchemoattraction chambers. Effect of NO on the adhesion and migration was assessed with or without co-exposure of S-nitroso-N-acetylpenicillamine (SNAP).
RESULTS
NO did not affect the degree of adhesion or migration of HTMC (p > 0.05). The degree of adhesion increased although the degree of migration decreased with 1% serum (p < 0.05). Degrees of migrations decreased after mechanical stress (p < 0.05).
CONCLUSIONS
NO may not affect the adhesion or migration of HTMC.

Keyword

Adhesion; Migration; Nitric oxide; Trabecular meshwork cells

MeSH Terms

Humans
Nitric Oxide
S-Nitroso-N-Acetylpenicillamine
Stress, Mechanical
Trabecular Meshwork
Nitric Oxide
S-Nitroso-N-Acetylpenicillamine

Figure

  • Figure 1. Effect of NO (Nitric oxide) donor on the production of NO. S-nitroso-N-acetylpenicillamine (SNAP) increased NO in a dose-dependent manner compared serum-free or 1% fetal bovine serum (FBS) (*p < 0.05).

  • Figure 2. Effect of NO (Nitric oxide) donor on the adhesion of trabecular meshwork cells. S-nitroso-N-acetylpenicillamine (SNAP) did not affect on the cell adhesion, but 1% fetal bovine serum (FBS) increased cellular adhesion significantly (*p < 0.05).

  • Figure 3. Effect of NO (Nitric oxide) donor on the migration of trabecular meshwork cells in normal condition. S-nitrosoN-acetylpenicillamine (SNAP) did not affect the cell migration, but 1% fetal bovine serum (FBS) decreased cellular migration significantly (*p < 0.05).

  • Figure 4. Effect of NO (Nitric oxide) donor on the migration of trabecular meshwork cells in mechanically stressed condition. After stress, the degree of cellular migration decreased in all groups and did not change significantly compared to non-exposed control (p > 0.05).


Reference

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