J Korean Ophthalmol Soc.  2011 Dec;52(12):1501-1506. 10.3341/jkos.2011.52.12.1501.

Effects of Mitomycin C on Eyelash-Regrowth in Epilated Rats

• Kim CR
• Yoo WS
• Kim KH
• Seo SW

• KMID: 2215156
• DOI: http://doi.org/10.3341/jkos.2011.52.12.1501

Abstract

PURPOSE
The aim of the present study was to determine the effects of mitomycin C (MMC) on eyelash regrowth in rats.
METHODS
Ten rats were subjected to bilateral eyelid epilation. One drop of 0.04% MMC solution and one drop of normal saline was then administered to the right and left eyelid, respectively. Three months post-epliation, the number of regrown eyelashes was counted, and a section of tissue from each eyelid was examined under light- and electron microscopies.
RESULTS
The number of regrown long eyelashes was 1.0 +/- 0.81 on the right, and 12.5 +/- 1.08 on the left. The rate of regrowth was 7.35 +/- 5.55% on the right and 98.45 +/- 3.27% on the left. Histological examination revealed no significant differences between the right and left eyelids, except for the absence of any hair shafts in the follicles on the right. However, electron microscopy revealed mitochondrial damage in the follicles of the right eyelid. These changes were not observed in tissue from the left eyelid.
CONCLUSIONS
The application of 0.04% MMC inhibited the regrowth of normal eyelashes in rats following simple epilation. These results suggest that MMC may be a novel therapy for replacing invasive surgery in trichiasis with no malpositioning.

Keyword

Eyelash; Mitomycin C; Trichiasis

MeSH Terms

Animals
Electrons
Eyelashes
Eyelids
Hair
Hair Removal
Microscopy, Electron
Mitomycin
Rats
Trichiasis
Mitomycin

Figure

• 

  Figure 1. Photographs of the eyes of an experimental rat. Long eyelashes of the left (A, B) & right (C, D) upper eyelids.(A) Prior to epilation. (B) 3 months post-epilation and administration of normal saline (eyelash-regrowth was observed). (C) Prior to epilation. (D) 3 months post-epilation and administration of 0.04% mitomycin C solution (eyelash-regrowth was not observed).

• 

  Figure 2. Light microscopy images of vertical section of excised rat eyelid tissue. The number and size of the hair follicle cells was normal in both the right and left eyelids. However, no hair shaft is present in the hair follicle on the right (treated with mitomycin C) (Hematoxylin-eosin, ×200). (A) Hair follicle of the left eyelid: 3 months post-epilation and administration of normal saline. (B) Hair follicle of the right eyelid: 3 months post-epilation and administration of 0.04% MMC solution.

• 

  Figure 3. Electron micrographs of the eyelid from rats. (A) Normal mitochondria are visible in the hair follicle of the left eyelid (saline-instilled). (B) Mitochondrial destruction is visible in the hair follicle of the right eyelid (mitomycin C-in-stilled). ∗ Mitochondria.

Reference

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