J Korean Surg Soc.  1998 Jan;54(1):23-27.

The Elevated Expression of the High Mobility Group-I(Y) Proteins in Thyroid Cancer using Semi-Quantitation RT-PCR

• Ryu JW
• Kim SJ

Abstract

High Mobility Group I(HMG-I) proteins are nuclear proteins that are required for induction of the human IFN-beta gene by virus and for the regulation of the tumor necrosis-beta factor and rRNA genes. Proteins I and Y result from alternative splicing of a single functional gene named HMGI(Y). In several studies, elevated expressions of the HMGI proteins (HMGI, HMGY, and HMGI-C) have been used as markers in thyroid cancer, but not in adenomas, goiters, and normal thyroid tissues and cells. Here, we try to demonstrate the elevated expression of the HMGI(Y) proteins in thyroid carcinomas by using semi-quantified RT-PCR (Reverse Transcription and Polymerase Chain Reaction). In cases of thyroid carcinomas 4 of 5(80%) were positive, in 10 cases of adenomas, goiters, and normal thyroid tissues, 1(10%) was positive. These results suggest that the semi-quantified RT-PCR is useful preoperative diagnostic tool for differentiating thyroid tumors.

Keyword

Thyroid cancer; HMGI(Y); Semi-quantified RT-PCR

MeSH Terms

Adenoma
Alternative Splicing
Genes, rRNA
Goiter
HMGA1a Protein
Humans
Nuclear Proteins
Thyroid Gland*
Thyroid Neoplasms*
HMGA1a Protein
Nuclear Proteins