J Korean Acad Prosthodont.  2014 Jul;52(3):165-176. 10.4047/jkap.2014.52.3.165.

The effects of desensitizing agents, bonding resin and tooth brushing on dentin permeability, in vitro

• Hong SW
• Park NJ
• Park YB
• Lee KW

• KMID: 2195419
• DOI: http://doi.org/10.4047/jkap.2014.52.3.165

Abstract

PURPOSE
The effects of desensitizing agent are often for a short duration. One of the reasons is believed to be wear of desensitizing agent by tooth brushing. To reduce the wear and make the duration longer, dental bonding resin was applied and the changes of dentin permeability after toothbrushing were measured.
MATERIALS AND METHODS
Extracted teeth free from caries were chosen. Coronal dentin discs with thickness of 1 mm were prepared. Using the split chamber device developed by Pashely, hydraulic conductance and scanning electron microscope images (SEM) were compared and contrasted before and immediately after the application of desensitizing agent and bonding resin and then after equivalent tooth brushing of 1 week, 2 weeks, and 6 weeks. Four commercially available desensitizing agents were used in this study; they were All-Bond 2, Seal & Protect, Gluma, and MS Coat. And Dentin/Enamel Bonding resin (Bisco Inc.) was used. The results of this study are as follows.
RESULTS
On all specimens, the hydraulic conductance decreased after the application of tooth desensitizing agent and bonding resin. Compared with the specimens treated only with desensitizer, the specimens treated with All-Bond 2, Gluma, MS Coat and plus D/E bonding resin had a little increase in hydraulic conductance after 1, 2 and 6-week tooth brushing. In case of Seal & Protect, the specimens showed the same result only after 6-week tooth brushing. On examination of SEM, the dentinal tubule diameter had decreased after treatment of desensitizing agents and bonding resin. And the specimens treated with All-Bond2, Seal&Protect, Gluma, MS Coat and plus D/E bonding resin had an significant decrease in diameter of dentinal tubule after 6-week tooth brushing.
CONCLUSION
According to the results of this study, it is effective to use bonding resin after application of desensitizer in reducing the wear by tooth brushing and making the duration longer. In this study, just 6-week tooth brushing was performed, and it is not enough to regard it as a long-term data. So further study is needed and more perfect method for treating dentin hypersensitivity should be developed.

Keyword

Dentin sensitivity; Gluma desensitizer; Tooth brushing; Dentin-Bonding agents

MeSH Terms

Dental Bonding
Dentin
Dentin Permeability*
Dentin Sensitivity
Dentin-Bonding Agents
Tooth*
Toothbrushing
Dentin-Bonding Agents

Figure

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  Fig. 1. Schematic view of Pashley's device used to measure hydraulic conductance of dentin disc.

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  Fig. 2. Photograph of Pashley's device used to measure hydraulic conductance of dentin disc (A); digital micrometer (B).

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  Fig. 3. Toothbrush abrasion testing machine.

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  Fig. 4. All-Bond® 2 and All-Bond® 2 + Bonding resin: % change values of hydraulic conductance.

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  Fig. 5. Seal & Protect® and Seal & Protect® + Bonding resin: % change values of hydraulic conductance.

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  Fig. 6. Gluma® and Gluma® + Bonding resin: % change values of hydraulic conductance.

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  Fig. 7. MS Coat® and MS Coat® + Bonding resin: % change values of hydraulic conductance.

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  Fig. 8. (A) All-Bond® 2: SEM of dentinal surface after agent treatment, (B) All-Bond® 2: SEM of dentinal surface after 6-week tooth brushing, (C) All-Bond® 2 + Bonding resin: SEM of dentinal surface after agent treatment, (D) All-Bond® 2 + Bonding resin: SEM of dentinal surface after 6-week tooth brushing.

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  Fig. 9. (A) Seal & Protect®: SEM of dentinal surface before agent treatment, (B) Seal & Protect®: SEM of dentinal surface after agent treatment, (C) Seal & Protect®: SEM of dentinal surface after 6-week tooth brushing, (D) Seal & Protect® + Bonding resin: SEM of dentinal surface after 6-week tooth brushing.

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  Fig. 10. (A) Gluma®: SEM of dentinal surface before agent treatment, (B) Gluma®: SEM of dentinal surface after agent treatment, (C) Gluma®: SEM of dentinal surface after 6-week tooth brushing, (D) Gluma® + Bonding resin: SEM of dentinal surface after 6-week tooth brushing.

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  Fig. 11. (A) MS Coat®: SEM of dentinal surface before agent treatment, (B) MS Coat®: SEM of dentinal surface after agent treatment, (C) MS Coat®: SEM of dentinal surface after 6-week tooth brushing, (D) MS Coat® + Bonding resin: SEM of dentinal surface after 6-week tooth brushing.

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