J Gynecol Oncol.  2008 Sep;19(3):162-168. 10.3802/jgo.2008.19.3.162.

Expression of the p16(INK4a) and Ki-67 in relation to the grade of cervical intraepithelial neoplasia and high-risk human papillomavirus infection

  • 1Women's Cancer Clinic, Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea.
  • 2Gynecologic Cancer Center, Department of Obstetrics and Gynecology, Kwandong University College of Medicine, Goyang, Korea. jwkim0630@kwandong.ac.kr
  • 3Department of Pathology, Kwandong University College of Medicine, Goyang, Korea.


The purposes of this study were to evaluate the expression of p16(INK4a) (referred as to p16) and Ki-67 in cervical intraepithelial neoplasia (CIN), and the correlation between high-risk human papillomavirus (HPV) infection and the above biomarkers. METHODS: We analyzed 31 patients who were diagnosed with CIN at Kwandong University Myongji Hospital from October 2006 to September 2007. CIN specimens (CIN1, 12; CIN2, 6; CIN3, 13) were obtained by colposcopy-directed biopsy (CDB) or loop electrical excision procedure (LEEP). The expressions of p16 and Ki-67 were evaluated by immunohistochemical methods with antibodies to p16 and Ki67. The immunohistochemical staining results were classified into four grades: 0, 1, 2 and 3. HPV genotyping or Hybrid Capture-II test was used to detect high-risk HPV. RESULTS: The expression of p16 (p<0.001) and Ki-67 (p=0.003) were positively associated with CIN grade. p16 expressions increased significantly with high-risk HPV infection (p=0.014), especially HPV type 16 and 58. Ki-67 expression was not related with high-risk HPV. There was positive correlation between the expression of the p16 and Ki-67 (p=0.007). CONCLUSION: CIN grade were positively related to the expression of p16 and Ki-67. p16 expressions of high-risk HPV specimens significantly increased more than Ki-67. Therefore, in the diagnosis of CIN and high-risk HPV infection, p16 can be a useful biomarker.


p16; Ki-67; HPV 16; HPV 58; Cervical intraepithelial neoplasia

MeSH Terms

Cervical Intraepithelial Neoplasia
Cyclin-Dependent Kinase Inhibitor p16
Human papillomavirus 16
Papillomavirus Infections
Cyclin-Dependent Kinase Inhibitor p16
Full Text Links
  • JGO
export Copy
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error