Ann Dermatol.  2011 Dec;23(Suppl 3):S393-S397. 10.5021/ad.2011.23.S3.S393.

The Use of Cultured Allogenic Keratinocyte Grafting in a Patient with Epidermolysis Bullosa Simplex

• Shin KC
• Park BY
• Kim HK
• Kim WS
• Bae TH

• KMID: 2171881
• DOI: http://doi.org/10.5021/ad.2011.23.S3.S393

Abstract

Epidermolysis bullosa (EB) is a rare genetic disease that is known for continuous skin blistering caused by minor trauma. The skin blisters and bullae that develop often cause skin defects. There is no definitive treatment for EB, only symptomatic relief. We report our experience with cultured allogenic keratinocyte grafting in a newborn patient with EB simplex who had unhealed raw surfaces and was not a skin grafting candidate. The skin lesions of the patient were covered with cultured allogenic keratinocyte grafts and re-epithelialized quickly with no scarring. Allogenic keratinocyte grafting reduced pain and produced noticeable improvements in the unhealed wounds. We think that allogenic keratinocyte grafting can play an important role in the management of patients with EB simplex.

Keyword

Epidermolysis bullosa simplex; Keratinocyte

MeSH Terms

Blister
Cicatrix
Epidermolysis Bullosa
Epidermolysis Bullosa Simplex
Humans
Infant, Newborn
Keratinocytes
Methylmethacrylates
Polystyrenes
Skin
Skin Transplantation
Transplants
Methylmethacrylates
Polystyrenes

Figure

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  Fig. 1 (A) Bleeding lesions (3×5 cm) on both feet of a 1 day-old boy who presented immediately after birth. (B) The medial aspects of both feet on post-birth day 12. (C) Epithelialization of the wounds on post-Kalloderm® application day 7.

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  Fig. 2 Loss of epidermal integrity occurred near the keratinocyte basal layer, which is the defining characteristic of epidermolysis bullosa simplex (H&E, ×400).

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  Fig. 3 The wounds were covered with Kalloderm® and petroleum roll gauze to reduce tissue trauma.

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