Biomol Ther.  2014 Mar;22(2):155-160.

A Comparison of the In Vitro Inhibitory Effects of Thelephoric Acid and SKF-525A on Human Cytochrome P450 Activity

• Song M
• Do H
• Kwon OK
• Yang EJ
• Bae JS
• Jeong TC
• Song KS
• Lee S

Abstract

Thelephoric acid is an antioxidant produced by the hydrolysis of polyozellin, which is isolated from Polyozellus multiplex. In the present study, the inhibitory effects of polyozellin and thelephoric acid on 9 cytochrome P450 (CYP) family members (CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) were examined in pooled human liver microsomes (HLMs) using a cocktail probe assay. Polyozellin exhibited weak inhibitory effects on the activities of all 9 CYPs examined, whereas thelephoric acid exhibited dose- and time-dependent inhibition of all 9 CYP isoforms (IC50 values, 3.2-33.7 muM). Dixon plots of CYP inhibition indicated that thelephoric acid was a competitive inhibitor of CYP1A2 and CYP3A4. In contrast, thelephoric acid was a noncompetitive inhibitor of CYP2D6. Our findings indicate that thelephoric acid may be a novel, non-specific CYP inhibitor, suggesting that it could replace SKF-525A in inhibitory studies designed to investigate the effects of CYP enzymes on the metabolism of given compounds.

Keyword

Thelephoric acid; Polyozellin; SKF-525A; Cytochrome P450; Non-specific inhibitor

MeSH Terms

Cytochrome P-450 CYP1A2
Cytochrome P-450 CYP2D6
Cytochrome P-450 CYP2E1
Cytochrome P-450 Enzyme System*
Humans
Hydrolysis
Metabolism
Microsomes, Liver
Proadifen*
Protein Isoforms
Cytochrome P-450 CYP1A2
Cytochrome P-450 CYP2D6
Cytochrome P-450 CYP2E1
Cytochrome P-450 Enzyme System
Proadifen
Protein Isoforms