Infect Chemother.  2011 Jun;43(3):240-244. 10.3947/ic.2011.43.3.240.

Clinical Efficacy Evaluation of Multi-parameter Real-time Polymerase Chain Reaction for the Central Venous Catheter-related Blood Stream Infection

• Kim B
• Park S
• Kim T
• Kim J
• Rim D
• Choi T
• Pai H
• Kang J

• KMID: 2170344
• DOI: http://doi.org/10.3947/ic.2011.43.3.240

Abstract

BACKGROUND
The study evaluated the clinical efficacy of a multi-parameter real-time polymerase chain reaction (PCR) test for patients with central venous catheter-related bloodstream infection (CRBSI).
MATERIALS AND METHODS
Thirty five patients suspected to have CRBSI were enrolled. The SeptiFast(TM) (SF) multi-parameter real-time PCR test (Roche Diagnostics, Germany) and blood culture were performed and results were compared.
RESULTS
The turn-around time for the SF test and blood culture was 32.6+/-28.9 hours and 115.8+/-23.5 hours, respectively. Among the 70 blood samples, the positive rates of SF test and blood culture were 34.3% and 27.1%, respectively, and the agreement rate was 62.9%. Gram-positive bacteria were detected in 10 patients with blood culture and 11 patients with SF test. Gram-negative bacteria were detected in one patient with a blood culture and in seven patients with SF test. Candida was not detected in blood culture but was detected in two patients by the SF test.
CONCLUSIONS
SF test was faster and more sensitive for the detection of blood pathogens than blood culture. It provides a more sensitive detection of gram-negative and Candida in blood than does blood culture testing.

Keyword

SeptiFast test; Catheter-related infections; Blood culture

MeSH Terms

Candida
Catheter-Related Infections
Gram-Negative Bacteria
Gram-Positive Bacteria
Humans
Real-Time Polymerase Chain Reaction
Rivers

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