J Korean Med Sci.  2006 Feb;21(1):90-94. 10.3346/jkms.2006.21.1.90.

Analysis of the Monocyte Chemoattractant Protein 1 -2518 Promoter Polymorphism in Korean Patients with Alopecia Areata

• Hong SB
• Jin SY
• Park HJ
• Jung JH
• Sim WY

• KMID: 2157789
• DOI: http://doi.org/10.3346/jkms.2006.21.1.90

Abstract

Monocyte chemoattractant protein-1 (MCP-1) levels are increased in scalp lesions of patients with alopecia areata (AA), suggesting a role in the development of AA. Recently, a biallelic A/G polymorphism in the MCP-1 promoter at position -2518 has been found, influencing the level of MCP-1 expression in response to an inflammatory stimulus. We investigated whether the presence of these polymorphisms were associated with AA in Korean population. 145 Korean patients with AA, 246 healthy subjects without clinical evidence of AA were screened for genotype with a PCR-based assay. In the AA patients the frequency of the A and G alleles was 40.3 and 59.7%, respectively and the distribution of the A/A, A/G and G/G genotypes was 19.3, 42.1 and 38.6%, respectively. Amongst the controls the frequency of the A and G alleles was 39.8 and 60.2%, and the distribution of the A/A, A/G, G/G genotypes in the same group was 17.5, 44.7 and 37.8%, respectively. There was no significant difference in the allele frequencies and genotype distributions between the patients and the controls (p=0.889, p=0.848, respectively). Our data indicates that no association exists between the -2518A/G polymorphism of the MCP-1 gene and susceptibility to alopecia areata.

Keyword

Alopecia Areata; Chemokines; MCP 1 Protein, human; Polymorphism, Genetic

MeSH Terms

Adolescent
Adult
Alleles
Alopecia Areata/*genetics
Case-Control Studies
Female
Gene Frequency
Genetic Predisposition to Disease/genetics
Genotype
Humans
Korea
Male
Middle Aged
Monocyte Chemoattractant Protein-1/*genetics
*Polymorphism, Single Nucleotide
Promoter Regions (Genetics)/*genetics

Reference

1. Benoit S, Toksoy A, Goebeler M, Gillitzer R. Selective expression of chemokine monokine induced by interferon-γ in alopecia areata. J Invest Dermatol. 2003. 121:933–935.

2. Tazi-Ahnini R, di Giovine FS, McDonagh AJ, Messenger AG, Amadou C, Cox A, Duff GW, Cork MJ. Structure and polymorphism of the human gene for the interferon-induced p78 protein (MX1): evidence of association with alopecia areata in the Down syndrome region. Hum Genet. 2000. 106:639–645.

3. Tak WJ, Chung YS, Ro BI. A clinical study on alopecia areata (1996-2000). Korean J Dermatol. 2002. 40:791–800.

4. Tazi-Ahnini R, Cork MJ, Gawkrodger DJ, Birch MP, Wengraf D, McDonagh AJ, Messenger AG. Role of the autoimmune regulator (AIRE) gene in alopecia areata: strong association of a potentially functional AIRE polymorphism with alopecia universalis. Tissue Antigens. 2002. 60:489–495.

5. McDonagh AJ, Messenger AG. Alopecia areata. Clin Dermatol. 2001. 19:141–147.

6. McElwee KJ, Hoffmann R. Alopecia areata-Animal models. Clin Exp Dermatol. 2002. 27:410–417.

7. Baggiolini M. Chemokines and leukocyte traffic. Nature. 1998. 392:565–568.

8. Noris M, Bernasconi S, Casiraghi F, Sozzani S, Gotti E, Remuzzi G, Mantovani A. Monocyte chemoattractant protein-1 is excreted in excessive amounts in the urine of patients with lupus nephritis. Lab Invest. 1995. 73:804–809.

9. Rovin BH, Rumancik M, Tan L, Dickerson J. Glomerular expression of monocyte chemoattractant protein-1 in experimental and human glomerulonephritis. Lab Invest. 1994. 71:536–542.

10. Harigai M, Hara M, Yashimura T, Leonard EJ, Inoue K, Kashiwazaki S. Monocyte chemoattractant protein-1 (MCP-1) in inflammatory joint diseases and its involvement in the cytokine network of rheumatoid synovium. Clin Immunol Immunopathol. 1993. 69:83–91.

11. Asano T, Ogawa S. Expression of MCP-1 in Kawasaki disease: the anti-inflammatory effect of gamma globulin-therapy. Scand J Immunol. 2000. 51:98–103.

12. Rovin BH, Lu L, Saxena R. A novel polymorphism in the MCP-1 gene regulatory region that influences MCP-1 expression. Biochem Biophys Res Commun. 1999. 259:344–348.

13. Jibiki T, Terai M, Shima M, Ogawa A, Hamada H, Kanazawa M, Yamamoto S, Oana S, Kohno Y. Monocyte chemoattractant protein-1 gene regulatory region polymorphism and serum levels of monocyte chemoattractant protein-1 in Japanese patients with Kawasaki disease. Arthritis Rheum. 2001. 44:2211–2212.

14. Aguilar F, Gonzalez-Escribano MF, Sanchez-Roman J, Nunez-Roldan A. MCP-1 promotor polymorphism in Spanish patients with systemic lupus erythematosus. Tissue Antigens. 2001. 58:335–338.

15. Szalai C, Duba J, Prohaszka Z, Kalina A, Szabo T, Nagy B, Horvath L, Csaszar A. Involvement of polymorphisms in the chemokine system in the susceptibility for coronary artery disease (CAD). Coincidence of elevated Lp (a) and MCP-1 -518 G/G genotype in CAD patients. Atherosclerosis. 2001. 158:233–239.

16. Price VH, Colombe BW. Heritable factors distinguish two types of alopecia areata. Dermatol Clin. 1996. 14:679–689.

17. Colombe BW, Price VH, Khoury EL, Garovoy MR, Lou CD. HLA class II antigen association help to define two types of alopecia areata. J Am Acad Dermatol. 1995. 33:757–764.

18. Tesch GH, Maifert S, Schwarting A, Ralling BJ, Kelley VR. Monocyte chemoattractant protein 1-dependent leukocytic infiltrates are responsible for autoimmune disease in MRL-Fas ^lprmice. J Exp Med. 1999. 12:1813–1824.

19. Zoja C, Liu XH, Donadelli R, Abbate M, Testa D, Corna D, Taraboletti G, Vecchi A, Dong QG, Rollins BJ, Bertani T, Remuzzi G. Renal expression of the monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol. 1997. 8:720–729.

20. Hwang SY, Cho ML, Park B, Kim JY, Kim YH, Min DJ, Min JK, Kim HY. Allelic frequency of the MCP-1 promoter -2518 polymorphism in the Korean population and in Korean patients with rheumatoid arthritis, systemic lupus erythematosus and adult-onset Still's disease. Eur J Immunogenet. 2002. 29:413–416.