J Korean Ophthalmol Soc.  1996 Jan;37(1):19-29.

The Effect of Ascorbic Acid and its Derivative on Cultured Rabbit Keratocytes

• Lee DY
• Oum BS

Abstract

The rabbit keratocytes were cultured to evaluate the effects of L-ascorbic acid(AA), ascorbic acid 2-sulfate(AA-2S), and ascorbic acid 2-phosphate(AA-2P) by measuring cell numbers and 3H-thymidine incorporation. AA at a concentration of 0.05mM enhanced the proliferation of the cells progressively. Addition of 0.1, and 0.5mM AA stimulated the proliferation of cells in the 3rd and 7th day after culture and inhibited in the 15th and 20th day in a dose-dependent manner. AA-2S showed a similar pattern to those of the AA effect, although the inhibitory effect was milder than AA. All concentrations of AA-2P enhanced the proliferation of the cells from the early phase. The effect was prominent in the late phase in a dose-dependent manner. These results suggest that AA-2P is the most stable and the least cytotoxic in the aqueous solution state or culture media and, 0.1-0.5mM concentration of it is best in the promotion of the proliferation of the cultured keratocytes.

Keyword

Ascorbic acid; Ascorbic acid 2-phosphate; Ascorbic acid 2-sulfate; Keratocytes

MeSH Terms

Ascorbic Acid*
Cell Count
Culture Media
Ascorbic Acid
Culture Media