Korean J Dermatol.  1987 Jun;25(3):299-308.

Separation of Human Epidermal Langerhans Cells by Density Gradient Centrifugation on a Colloidal Silica ( Percoll ) Gradient Method and Autologous , Allogeneic Mixed Skin Cell Leukocyte Culture Reactions

• Bae YJ
• Haw CR

Abstract

Separation of Langerhans cells in epidermis of 16 healthy Korean individuals were performcd. Separation of Langerhans cells by density gradient centrifugation on a colloidal sillica(percoll) polyvinilpyrrolidone gradient. And autologous, allogeneic mixed skin cell leukocyte culture reaction was done with each fractionatcd cpidermal cell suspensions. Also lymphocytes, epidermal cells was cultured in media alone, respectively. The results was quantitated by the incorporation of H-thymidine by p-liquid scintillation counter. The densities of I angerhans cells within the epidermal cells, fraction-2 was most higher concentration (22.0+2.8%) and fraction-5 was most lower concentration (3.4+ l.9%). 2. In the comparison of the results of Langehans cells enriched and depleted population in autologous mixed skin cell leukocyte culture reaction, the former was higher than the latter on lymphocyte stimulatory capacity. There was significant differences(p<0.005) And also same as result in allogeneic mixed skin cell leukocyte culture reaction. 3. Langerhans cells enriched fraction in this study was more lymphocyte stimulatory capacity than depleted fraction in allogeneic mixed skin cell leukocyte culture(p<0.01~0.05). Ailogeneic mixed skin cell leukocyte culture reaction was more lymphocyte stimulatory capacity than the autologous(p<0.005~0.05).

Keyword

Langerhans cells; Density gradient; Percoll; Autologous; Allogeneic mixed skin cell leukocyte culture reaction

MeSH Terms

Centrifugation, Density Gradient*
Colloids*
Epidermis
Humans*
Langerhans Cells*
Leukocytes*
Lymphocytes
Scintillation Counting
Silicon Dioxide*
Skin*
Suspensions
Colloids
Silicon Dioxide
Suspensions