J Korean Assoc Maxillofac Plast Reconstr Surg.
2009 May;31(3):198-206.
Mandibular bone regeneration using autogenous skin-derived precursor cells with a mixed demineralized bone and fibrin glue scaffold in miniature pigs
- Affiliations
-
- 1Department Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University, Korea. parkbw@gsnu.ac.kr
- 2Department of Oral & Maxillofacial Surgery, School of Dentistry, Pusan National University, Korea.
Abstract
-
PURPOSE: The aims of this study were to assess the in vitro co-culturing pattern of isolated skin-derived precursor cells (SKPs) with a mixed demineralized bone (DMB) and fibrin glue scaffold and to evaluate in vivo osteogenesis after transplantation of autogenous SKPs with a these mixed scaffold in the animal's mandibular defects.
MATERIALS AND METHODS
We isolated SKPs from the ears of adult 4 miniature pigs. The isolated SKPs were co-cultured with a mixed DMB and fibrin glue scaffold in a non-osteogenic medium for 1, 2, and 4 weeks. Histological characteristics of in vitro co-cultured cells and scaffold were evaluated. 1x107 cells/100 microl of autogenous porcine SKPs were grafted into the mandibular defects with a DMB and fibrin glue scaffold. In the control sites, only a scaffold was grafted, without SKPs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies.
RESULTS
Homogeneously shaped skin-derived cells were isolated from porcine ear skin after 3 or 4 weeks of primary culture. In vitro osteogenic differentiation of SKPs was observed after co-culturing with a DMB and fibrin glue scaffold in a non-osteogenic medium. Von Kossa-positive bone minerals were also noted in the co-cultured medium at 4 weeks. As the culture time progressed, the number of observable cells increased. Trabecular new bone formation and osteocalcin expression were more pronounced in the SKP-grafted group compared to the control group.
CONCLUSION
These findings suggest that autogenous SKP grafting with a DMB and fibrin glue scaffold can serve as a useful alternative to bone grafting technique.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Maxillary sinus floor elevation using autogenous skin-derived mesenchymal stem cells in miniature pigs
- A Comparative Study on Regeneration of Bone Defects after the Grafts of Demineralized Bone Matrix and Hydroxyapatite
- A Double Layers Technique for Maxillary Sinus Augmentation with Demineralized and Mineralized Bone Graft Materials
- Effects of fibrin glue on bone formation in combination with deproteinized bone xenografts in humans
- Bone tissue engineering using PLLA/HA composite scaffold and bone marrow mesenchymal stem cell
