J Korean Ophthalmol Soc.  2000 May;41(5):1068-1072.

Carbonic Anhyd rase Activity in Muller Cell

• Cho NC
• Kweon EY

Abstract

Carbonic anhydrase, an enzyme catalysing the reversible hydration of carbon dioxide, is present in the Muller cells.Because the enzyme is not present in other uroretinal cells in the retina, it can be used as a marker for Muller cells.Carbonic anhydrase activity was demonstrated bnzymehistochemically in human and rabbit Muller cells to know a relation of metabolic functions and carbonic anhydrase activity.Human retinas were obtained from donor eyes.The eyes were enucleated immediately after death forenzymatic activity. In human retina, heavy staining was found in the inner nuclear layer and nerve fiber layer, moderate staining in the outer nuclear layer and weak or no staining in the plexiform layers.In rabbit retina, heavy staining was found in the nerve fiber layer and nuclear layers and weak reaction in the two plexiform layers. These findings suggest that Muller cells may participate in CO2 homeostasis mechanism of carbonic anhydrase in the retina.

Keyword

Carbonic anhydrase; Muller cell; Metabolism

MeSH Terms

Carbon Dioxide
Carbon*
Carbonic Anhydrases
Ependymoglial Cells
Homeostasis
Humans
Metabolism
Nerve Fibers
Retina
Tissue Donors
Carbon
Carbon Dioxide
Carbonic Anhydrases