Clin Exp Otorhinolaryngol.  2015 Sep;8(3):268-274. 10.3342/ceo.2015.8.3.268.

A Higher Angiogenin Expression is Associated With a Nonnuclear Maspin Location in Laryngeal Carcinoma

• Lovato A
• Lionello M
• Staffieri
• Blandamura S
• Tealdo G
• Giacomelli L
• Staffieri
• Marioni G

• KMID: 2117523
• DOI: http://doi.org/10.3342/ceo.2015.8.3.268

Abstract

OBJECTIVES
In numerous malignancies, angiogenin (ANG) and Maspin are important proangiogenic and antiangiogenic regulators, respectively. The aim of this study was to identify potential relationships between the biological roles of these two proteins in laryngeal squamous cell carcinoma (LSCC).
METHODS
Immunohistochemical staining for ANG and Maspin was performed on specimens from 76 consecutive LSCC patients treated with surgery alone, considering the subcellular pattern of Maspin expression. Univariate and multivariate statistical models were used for prognostic purposes.
RESULTS
On univariate analysis, a different level of ANG expression was seen for patients stratified by subcellular Maspin expression pattern: the mean ANG expression was higher in cases with a nonnuclear MASPIN expression than in those with a nuclear pattern (P=0.002). Disease-free survival (DFS; in months) differed significantly when patients were stratified by N stage (P=0.01). Patients whose Maspin expression was nonnuclear (i.e., it was cytoplasmic or there was none) had a significantly higher recurrence rate (P<0.001), and shorter DFS (P=0.01) than those with a nuclear Maspin pattern. The mean ANG expression was significantly higher in cases with loco-regional recurrent disease (P=0.007); and patients with an ANG expression > or =5.0% had a significantly shorter DFS than those with an ANG expression <5.0% (P=0.007). On multivariate analysis, ANG expression > or =5.0% was a significant, independent, negative prognostic factor in terms of DFS (P=0.041).
CONCLUSION
Our results support the hypothesis that a higher ANG expression is associated with a nonnuclear Maspin expression pattern in patients with LSCC. Further studies are needed to clarify the relationship between the ANG and Maspin pathways, and their potential diagnostic and therapeutic role in LSCC.

Keyword

Laryngeal Neoplasms; Angiogenin; SERPIN-B5; Intracellular Space; Prognosis

MeSH Terms

Carcinoma, Squamous Cell
Cytoplasm
Disease-Free Survival
Humans
Intracellular Space
Laryngeal Neoplasms
Models, Statistical
Multivariate Analysis
Prognosis
Recurrence

Figure

• 

  Fig. 1 (A, B) A patient whose laryngeal squamous cell carcinoma recurred, with a high angiogenin (ANG) expression (A), and a cytoplasmic pattern of Maspin expression (B), in carcinoma cells; (C, D) a patient who had no evidence of disease during the follow-up, with a low ANG expression (C), and a nuclear pattern of Maspin expression (D). (A-D) Immunohistochemical staining.

• 

  Fig. 2 (A) Disease-free survival estimates by angiogenin (ANG) expression (%) in laryngeal carcinoma cells (ANG<5.0% or ≥5.0%); (B) disease-free survival estimates by pattern of Maspin expression in laryngeal carcinoma cells (nuclear or nonnuclear); time calculated in months.

• 

  Fig. 3 Square root transformed box plot showing the different angiogenin (ANG) expression in patients stratified by subcellular Maspin expression pattern: mean ANG expression was higher in patients with a nonnuclear Maspin expression pattern (nonnuclear) than in those with a nuclear pattern (nuclear).

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