J Korean Surg Soc.
1999 Jun;56(6):848-860.
Phospholipase D, MMP-2 and TIMP-2 Expression in the Human Colorectal Cancer
- Affiliations
-
- 1Department of General Surgery, Ulsan University College of Medicine, Ulsan University Hospital, Ulsan, Korea.
- 2Department of Pathology, Ulsan University College of Medicine, Ulsan University Hospital, Ulsan, Korea.
- 3Department of Internal Medicine, Ulsan University College of Medicine, Ulsan University Hospital, Ulsan, Korea.
Abstract
-
BACKGROUND: Tumor invason and metastasis are the major causes of morbidity and death for cancer patients. Metastatic spread depends critically upon the invasiveness of the tumor cells, i.e., their ability to breach basement membrane by profusely secreting specific proteolytic enzymes such as MMP-2. TIMP-2 has a high affinity for progelatinase A and will form a 1:1 complex with either the latent or activated forms of the enzyme and has inhibitory activity against MMP-2. Laminin induced activation of Phospholipase D (PLD) and consequent generation of phosphatidic acid are involved in a signal propagation pathway leading to induction of MMP-2 in metastatic HT 1080 fibrosarcoma cells. We also studied a expression of PLD, MMP-2 and TIMP-2 in colorectal adenocarcinoma.
METHODS
Colorectal adenocarcinomas from 13 patients in our hospital were studied for immunohistochemical expression of PLD, MMP-2, and TIMP-2 to assess their diagnostic and prognostic importance as well as relation between PLD and MMP-2.
RESULTS
1) Expression of PLD-2 was detected in 77% of the cases in colorectal adenocarcinomas. 2) MMP-2 expression was significantly associated with the presence of lymph-node metastasis, with moderated to strong expression present in 100% of the cases compared with 28.6% of the non-metastatic cases (P-value=0.017). 3) For colorectal adenocarcinomas, a strong correlation between PLD and MMP-2 expression was detected (P-value=0.008).
CONCLUSION
PLD-2 can be used as a potential marker for malignant disease in colorectal adenocarcinomas. MMP-2 expression was significantly associated with the presence of lymph-nodemetastasis. A strong correlation between PLD and MMP-2 expression was also detected in colorectal carcinoma.
Keyword
MeSH Terms
-
Adenocarcinoma
Basement Membrane
Colorectal Neoplasms*
Fibrosarcoma
Humans*
Laminin
Neoplasm Metastasis
Peptide Hydrolases
Phosphatidic Acids
Phospholipase D*
Phospholipases*
Tissue Inhibitor of Metalloproteinase-2*
Laminin
Peptide Hydrolases
Phosphatidic Acids
Phospholipase D
Phospholipases
Tissue Inhibitor of Metalloproteinase-2
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Significance of Increased Expression of the Tissue Inhibitor of Matrix Metalloproteinase-2 in Human Colorectal Carcinomas
- Expression of MMP-2 (Matrix Metalloproteinase Type 2) and TIMP-2 (Tissue Inhibitor of Metalloproteinase Type 2) in Color
- Expressions of Phospholipase D, MMP-2, and TIMP-2 in Adenocarcinomas of the Lung
- Clinical significance of the serum levels of matrix metalloproteinases and the tissue inhibitors of metalloproteinases for gastric and colorectal cancer patients
- Immunodetections of the Metalloproteinase (MMP-2 and MMP-9) and Tissue Inhibitor of Metalloproteinases (TIMP-2) in Prostatic Adenocarcinomas
