Korean J Gastroenterol.  1999 Jun;33(6):786-798.

Comparison between Clinical Laboratory Data and Degree of Stain for Type ?Collagen, Laminin, Fibronectinin Li ver Biopsy Specimens in Patients with Hepatitis B Virus Induced Chronic Liver Disease

• Kim JB
• Park GT
• Hwang SH
• Park MS
• Choi HS
• Yoon BC
• Ham JS
• Kee CS
• Park KN
• Hong EK
• Park MH
• Lee MH

Abstract

BACKGROUND/AIMS: Hepatic fibrosis is caused by increased deposition of liver connective tissue components in the intercellular space. This leads to disturbance of intrahepatic blood flow and hind rance of exchange process between blood and cells. We investigated the deposition of extracellula matrix in the liver of patient with HBsAg positive chronic liver disease and the results were compared with those of other liver function tests.
METHODS
Needle-biopsied specimen was obtained from 31 patients with mild chronicactive hepatitis (CAH), 30 patients with moderate CAH, 19 pati ents with severe CAH, and 18 patients with cirrhosis. Immunohistochemical stains using antibodies were performed for type ?collagen, fibronectin and laminin.
RESULTS
Shunt index which could be measured with Thallium 201 scan was significantly increased according to the degree of stain for type ?collagen in portal area, especially in severe chronic active hepatitis and cirrhosis group. Pro thrombin time (%) and serum albumin were decreased according to the degree of stain for laminin in lobule.
CONCLUSIONS
These results suggest that the deposit of type ?collagen in portal area i a major contributing factor in the development of intrahepatic shunt and the deposit of laminin within Disse' s space may cause impairment of the hepatic function in cirrhosis.

Keyword

Type ?collagen; Laminin; Immunohistochemical stain; Chronic HBV hepatitis

MeSH Terms

Antibodies
Biopsy*
Collagen*
Coloring Agents
Connective Tissue
Extracellular Space
Fibronectins
Fibrosis
Hepatitis B Surface Antigens
Hepatitis B virus*
Hepatitis B*
Hepatitis*
Hepatitis, Chronic
Humans
Laminin*
Liver Diseases*
Liver Function Tests
Liver*
Serum Albumin
Thallium
Thrombin Time
Antibodies
Collagen
Coloring Agents
Fibronectins
Hepatitis B Surface Antigens
Laminin
Serum Albumin
Thallium