Korean J Med.  1999 Mar;56(3):359-366.

Linkage between gene marker of chromosome 11q13 and IgE - receptor mediated basophil histamine release

• Kim YK
• Cho SH
• Koh YY
• Son JW
• Min KU
• Kim YY

Abstract

OBJECTIVE
To evaluate that genetic factor(s) in chromosome 11q13 may control the expression of basophil histamine release (BaHR) after anti-IgE stimuli, linkage analysis between this phenotype and gene marker of chromosome 11q13 was performed. Material and methods: BaHR after anti-IgE stimuli and genotyping chromosome 11q13 using microsatellite marker (D11S97) was performed in 56 probands with asthma and 59 their sibs. The linkage between the phenotype and the genotype was evaluated by affected and quantitative trait locus (QTL) sib-pair analysis.
RESULTS
Maximal BaHR after anti-IgE were 43.3+/-3.5% in probands and 29.5+/-2.6% in sibs, respectively. Of 20 sib-pairs with the maximal BaHR more than 33%, 11 (55%) shared two D11S97 alleles, 9 (45%) shared one allele, neither sib-pair shared identical allele. The sharing rate of D11S97 alleles was 75.5%, which indicates linkage of the phenotype and the genotype (p= 0.026). Difference of the maximal BaHR between probands and their sibs was smaller in sib-pairs with two identical alleles than in those with one identical allele and with no identical allele (14.1+/-2.5% vs. 25.8+/-3.1% vs. 40.9+/-4.9%).
CONCLUSION
Expression of basophil histamine release after anti-IgE stimuli was linked to gene marker of chromosome 11q13.

Keyword

basophil histamine release; chromosome 11q13; asthma; IgE-receptor; linkage analysis

MeSH Terms

Alleles
Asthma
Basophils*
Genotype
Histamine Release*
Histamine*
Immunoglobulin E*
Microsatellite Repeats
Phenotype
Quantitative Trait Loci
Histamine
Immunoglobulin E