Korean J Physiol Pharmacol.  2015 Nov;19(6):543-547. 10.4196/kjpp.2015.19.6.543.

Combined Skin Moisturization of Liposomal Serine Incorporated in Hydrogels Prepared with Carbopol ETD 2020, Rhesperse RM 100 and Hyaluronic Acid

Affiliations
  • 1College of Pharmacy, Chung-Ang University, Seoul 06974, Korea. jaehwi@cau.ac.kr
  • 2College of Medicine, Chung-Ang University, Seoul 06974, Korea.
  • 3Department of Pharmaceutical Industry Management, Chung-Ang University, Seoul 06974, Korea.
  • 4Department of Food Science and Technology, Chung-Ang University, Anseong 17546, Korea.
  • 5Erom Co., Ltd., Chuncheon 24441, Korea.
  • 6Graduate School of Food and Drug Administration, Chung-Ang University, Seoul 06974, Korea.

Abstract

We investigated the combined moisturizing effect of liposomal serine and a cosmeceutical base selected in this study. Serine is a major amino acid consisting of natural moisturizing factors and keratin, and the hydroxyl group of serine can actively interact with water molecules. Therefore, we hypothesized that serine efficiently delivered to the stratum corneum (SC) of the skin would enhance the moisturizing capability of the skin. We prepared four different cosmeceutical bases (hydrogel, oil-in-water (O/W) essence, O/W cream, and water-in-oil (W/O) cream); their moisturizing abilities were then assessed using a Corneometer(R). The hydrogel was selected as the optimum base for skin moisturization based on the area under the moisture content change-time curves (AUMCC) values used as a parameter for the water hold capacity of the skin. Liposomal serine prepared by a reverse-phase evaporation method was then incorporated in the hydrogel. The liposomal serine-incorporated hydrogel (serine level=1%) showed an approximately 1.62~1.77 times greater moisturizing effect on the skin than those of hydrogel, hydrogel with serine (1%), and hydrogel with blank liposome. However, the AUMCC values were not dependent on the level of serine in liposomal serine-loaded hydrogels. Together, the delivery of serine to the SC of the skin is a promising strategy for moisturizing the skin. This study is expected to be an important step in developing highly effective moisturizing cosmeceutical products.

Keyword

Corneometer; Hydrogel; Liposome; Serine; Skin moisturization

MeSH Terms

Hyaluronic Acid*
Hydrogel*
Hydrogels*
Liposomes
Serine*
Skin*
Water
Hyaluronic Acid
Hydrogel
Hydrogels
Liposomes
Serine
Water

Figure

  • Fig. 1 Effect of formulation type on moisture content of the skin (A). Changes in moisture content of the skin as a function of time (B) are plotted to assess the moisture hold capacity of the skin by calculating the area under the moisture content change-time curve. Mean±SD (n=40~50).

  • Fig. 2 Effect of serine, blank liposomes, and hydrogel loaded with liposomal serine on the moisture content of the skin. The profiles of changes in the skin moisture contents were produced after monitoring the moisture content values of the skin measured at 0, 0.5, 1, 1.5, 2, 2.5 and 3 h of the experiments. Mean±SD (n=40~50).

  • Fig. 3 Effect of serine levels in the liposomes incorporated in hydrogel bases on moisture content of the skin. The profiles of changes in skin moisture contents were produced after monitoring the moisture content values of the skin measured at 0, 0.5, 1, 1.5, 2, 2.5 and 3 h of the experiments. Mean±SD (n=40~50).


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